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From 2010.igem.org

Andreas

Sequencing results

Sequencing results from 27/9 samples arrived and were analyzed by nucleotide BLAST.

Construct	Sequencing result	Blastn result	Comment
pEX.N-TAT⋅SOD⋅His 3	pEX.nTAT.SOD.his 3_premix	Blastn	Verified. Three silent mutations in His tag; does not alter a.a. sequence.
pEX.N-TAT⋅SOD⋅His 4	pEX.nTAT.SOD.his 4_premix	Blastn	Not verified. Incorrect sequence.
pSB1A2.RBS.yCCS 3	pSB1A2.RBS.yCCS 3_premix	Blastn	Partly verified. Three mutations found: (1) Single base deletion in yCCS sequence, causing frame shift; (2) Insertion between SpeI and PstI, not affecting expression of cloning; (3) Point mutation in PstI site, disabling PstI restriction.
pSB1A2.RBS.yCCS 4	VF2	pA.RBS.yCCS4_VF2
pSB1K3.N-TAT⋅SOD⋅His 4	VF2	pK.nTAT*SH4_VF2
pSB1K3.N-TAT⋅SOD⋅His 5	VF2	pK.nTAT*SH5_VF2
pSB1K3.N-Tra10⋅SOD⋅His 5	VF2	pK.nTra10*SH5_VF2
pSB1K3.N-LMWP⋅SOD⋅His 1	VF2	pK.nLMWP*SH1_VF2
pSB1C3.N-LMWP⋅SOD⋅His 1	VF2	pC.nLMWP*SH1_VF2
pSB1C3.N-LMWP⋅SOD⋅His 4	VF2	pC.nLMWP*SH4_VF2
pEX.SOD	pEX.SOD_premix	Blastn	Verified. Insertion found between SpeI and PstI; does not affect expression.
pEX.yCCS 5	pEX.yCCS 5_premix	Blastn	Verified. Insertion found between SpeI and PstI; does not affect expression.
pEX.SOD⋅His	pEX.SOD.his_premix	Blastn	Verified. Three silent mutations in His tag; does not alter a.a. sequence.
pEX.His⋅SOD	pEX.his.SOD_premix	Blastn	Verified. Three silent mutations in His tag; does not alter a.a. sequence.