Revision as of 09:29, 6 July 2010

Andreas

Testing Top10 competent cells and antibiotic agar plates

Results from 22/6

Transformed plasmid	Plate	Growth
pEX	100 ug/ml Amp	Yes
None	100 ug/ml Amp	No
pEX	25 ug/ml Cm	No
None	25 ug/ml Cm	No

Growth on Amp plate indicated transformation competence. No growth on other plates indicated good antibiotic selection.

Note: The transformed pEX vector was thought to carry a Cm resistance cassette insert. This was, however, discovered to be wrong.

Plasmid preparation of pEX vector

Two clones picked and each inoculated in 5 ml LB with Amp and grown ON in 37°C, 250 rpm.

Transformation with pMA vector

The pMA (BBa_K157000) vector could become useful for cloning and construction of our parts, as it carries the complete Freiburg prefix and suffix sequences (in contrast to the standard BioBrick vectors pSB1x3).

Transformed Top10 cells with pMA vector carrying a Freiburg fusion His tag (BBa_K157011) insert. Cells plated on 100 ug/ml Amp plates.

Hassan

Started to use softwares for making 3D animations and/or movies, started by making protein A and IgG Protease.

softwares used:

Molecular Maya
Chimera

@@ Line 39: / Line 39: @@
 The pMA (BBa_K157000) vector could become useful for cloning and construction of our parts, as it carries the complete Freiburg prefix and suffix sequences (in contrast to the standard BioBrick vectors pSB1x3).
-* Top10 cells was transformed with the pMA vector carrying a Freiburg fusion His tag (BBa_K157011) insert. Cells grown on 100 ug/ml Amp plates.
+* Transformed Top10 cells with pMA vector carrying a Freiburg fusion His tag (BBa_K157011) insert. Cells plated on 100 ug/ml Amp plates.
 =Hassan=

Team:Stockholm/23 June 2010

From 2010.igem.org