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Team:Stockholm/22 July 2010
From 2010.igem.org
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| + | ==Johan== | ||
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| + | ===PCR=== | ||
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| + | The PCR samples that was run on a gel to verify that the colonies had been successfully site-directed mutagenesised seemed to be lost, and a new colony PCR was performed to verify the site-directed mutagenesis before cleaving and adding it to the target vectors. | ||
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| + | [[Image:Agarose Johan bFGF 22july 22july.jpg]] | ||
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| + | The ladder is FastRuler DNA ladder low range, but the bands does not show the expected size. Did I mistakenly use the FastRuler DNA ladder middle range? | ||
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Hassan
tyrp1 nalp1 mitf CGRP S100B foxd3 GGA1 LMP7 TAPBP
Johan
PCR
The PCR samples that was run on a gel to verify that the colonies had been successfully site-directed mutagenesised seemed to be lost, and a new colony PCR was performed to verify the site-directed mutagenesis before cleaving and adding it to the target vectors.
The ladder is FastRuler DNA ladder low range, but the bands does not show the expected size. Did I mistakenly use the FastRuler DNA ladder middle range?
