"
Team:Stockholm/1 July 2010
From 2010.igem.org
(Difference between revisions)
(→Transformations) |
|||
| Line 17: | Line 17: | ||
== Transformations == | == Transformations == | ||
| - | Several different transformations were performed. Top10 cells were transformed with pSB1x3 BioBrick vectors. BL21(DE3) cells were transformed with SOD- and yCCS-carrying pEX vectors as well as with the pMA vector to test competence: | + | Several different transformations were performed. Top10 cells were transformed with pSB1x3 BioBrick vectors and grown on agar plates with appropriate antibiotic(s). BL21(DE3) cells were transformed with SOD- and yCCS-carrying pEX vectors as well as with the pMA vector to test cell competence and grown on Amp plates: |
==== Top10 ==== | ==== Top10 ==== | ||
*pSB1A3.BBa_J04450 | *pSB1A3.BBa_J04450 | ||
Revision as of 22:19, 1 July 2010
| Home | Project Idea | Lab Work | Results | Modelling | Team | Follow us on  and 
| 2010.igem.org 
|
Contents |
Andreas
Preparation of competent BL21(DE3) cells
Competent cells were prepared as previously described.
- Modifications
- Growth temperature was changed to 37°C
- Final OD(600) was 0.57
Preparation of LB agar plates with antibiotics
- 50 ug/ml Km (20 plates)
- 50 ug/ml Amp & 25 ug/ml Cm (10 plates)
- 50 ug/ml Amp & 25 ug/ml Km (10 plates)
Plates were tested by plating with competent, non-transformed Top10 cells
Transformations
Several different transformations were performed. Top10 cells were transformed with pSB1x3 BioBrick vectors and grown on agar plates with appropriate antibiotic(s). BL21(DE3) cells were transformed with SOD- and yCCS-carrying pEX vectors as well as with the pMA vector to test cell competence and grown on Amp plates:
Top10
- pSB1A3.BBa_J04450
- pSB1C3.BBa_J04450
- pSB1K3.BBa_J04450
- pSB1AC3.BBa_J04450
- pSB1AK3.BBa_J04450
BL21(DE3)
- pEX.SOD
- pEX.yCCS
- pMA.BBa_K157011
Isolation of requested parts
Our three requested parts BBa_J18930 (mCerulean; CFP), BBa_J18931 (mCitrine; YFP) and BBa_J18932 (mCherry; RFP) arrived as agar stabs. These were plated onto Amp plates according to Registry instructions.
