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Team:Stockholm/14 October 2010
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(New page: {{Stockholm/Top2}} ==Andreas== ===Growth curve assay=== New attempt to measure growth curves for CPPs. ====Induction==== *150 ml side-arm E-flasks *15 ml LB *150 μl ON culture (1:100) ...) |
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Experiment was canceled after 270 min as I was made aware that the spectrophotometer only makes accurate measurements up to ≈OD<sub>590</sub> = 0.5. | Experiment was canceled after 270 min as I was made aware that the spectrophotometer only makes accurate measurements up to ≈OD<sub>590</sub> = 0.5. | ||
| + | |||
| + | ====ON cultures==== | ||
| + | New ON cultures set (including two for Mimmi) | ||
| + | *5 ml LB + Amp 100; 37 °C, 225 rpm | ||
| + | **BL21, pEX.SOD⋅His | ||
| + | **BL21, pEX.nTra10⋅SOD⋅His | ||
| + | **BL21, pEX.nTAT⋅SOD⋅His | ||
| + | **BL21, pEX.nLMWP⋅SOD⋅His | ||
| + | **(BL21, pEX.SOD) | ||
| + | **(BL21, pEX.yCCS) | ||
Revision as of 17:16, 14 October 2010
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Contents |
Andreas
Growth curve assay
New attempt to measure growth curves for CPPs.
Induction
- 150 ml side-arm E-flasks
- 15 ml LB
- 150 μl ON culture (1:100)
- BL21
- ON cultures set by Mimmi 13/10
- 37 °C, 220 rpm
OD590 measurements
| 0 min | 30 min | 60 min | 90 min | 120 min | 150 min | 180 min | 210 min | 240 min | 270 min | |
|---|---|---|---|---|---|---|---|---|---|---|
| pEX.SOD⋅His | 0.03 | 0.04 | 0.10 | 0.23 | 0.45 | 0.78 | 1.06 | 1.36 | 1.56 | 1.67 |
| pEX.nTra10⋅SOD⋅His | 0.03 | 0.04 | 0.09 | 0.19 | 0.36 | 0.61 | 0.94 | 1.18 | 1.42 | 1.56 |
| pEX.nTAT⋅SOD⋅His | 0.03 | 0.05 | 0.10 | 0.23 | 0.49 | 0.78 | 1.06 | 1.35 | 1.55 | 1.66 |
| pEX.nLMWP⋅SOD⋅His | 0.04 | 0.05 | 0.11 | 0.25 | 0.52 | 0.88 | 1.14 | 1.40 | 1.60 | 1.69 |
Experiment was canceled after 270 min as I was made aware that the spectrophotometer only makes accurate measurements up to ≈OD590 = 0.5.
ON cultures
New ON cultures set (including two for Mimmi)
- 5 ml LB + Amp 100; 37 °C, 225 rpm
- BL21, pEX.SOD⋅His
- BL21, pEX.nTra10⋅SOD⋅His
- BL21, pEX.nTAT⋅SOD⋅His
- BL21, pEX.nLMWP⋅SOD⋅His
- (BL21, pEX.SOD)
- (BL21, pEX.yCCS)
