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Team:Stockholm/11 August 2010
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== Andreas == | == Andreas == | ||
| + | |||
| + | ===Cloning of IgG protease=== | ||
| + | |||
| + | ''Continued from 10/8'' | ||
| + | |||
| + | ====Colony PCR==== | ||
| + | Four clones picked from each of the two plates (Q for quick transformation; S for standard transformation). | ||
| + | |||
| + | *QA, QB, QC, QD | ||
| + | *SA, SB, SC, SD | ||
| + | *Pos. control: pSB1A3.BBa_J04450 (plasmid) | ||
| + | |||
| + | '''Procedures'''<br> | ||
| + | As described in colony PCR protocol. Elongation time: 1:40. | ||
| + | |||
| + | ====Gel verification==== | ||
| + | [[image:ColPCR_IgGp_11aug.png|200px|thumb|right|'''Colony PCR verification of IgGp clones.'''<br>1 %, 80 V, 45 min.<br>'''Loading:''' 1 kb λ, QA, QB, QC, QD, SA, SB, SC, SD, Pos. contr.]] | ||
| + | 1 %, 80 V, 45 min | ||
| + | |||
| + | Expected: 1262 bp (BBa_J04450: 1386 bp) | ||
| + | |||
| + | '''Results''' | ||
| + | |||
| + | Very strangely sized bands again! None of the bands corresponding to the 1262 bp expected. Control corresponding well to expected 1386 bp. | ||
| + | |||
| + | Digested vector and insert will be tested on gel tomorrow. | ||
Revision as of 14:48, 12 August 2010
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Contents |
Hassan
- Started making animation for what's happening in our project.
- TODO for tomorrow: complete the interaction map, and starting summarizing texts for the map, hope to finish it by sunday...
Andreas
Cloning of IgG protease
Continued from 10/8
Colony PCR
Four clones picked from each of the two plates (Q for quick transformation; S for standard transformation).
- QA, QB, QC, QD
- SA, SB, SC, SD
- Pos. control: pSB1A3.BBa_J04450 (plasmid)
Procedures
As described in colony PCR protocol. Elongation time: 1:40.
Gel verification
1 %, 80 V, 45 min.
Loading: 1 kb λ, QA, QB, QC, QD, SA, SB, SC, SD, Pos. contr.
1 %, 80 V, 45 min
Expected: 1262 bp (BBa_J04450: 1386 bp)
Results
Very strangely sized bands again! None of the bands corresponding to the 1262 bp expected. Control corresponding well to expected 1386 bp.
Digested vector and insert will be tested on gel tomorrow.
