http://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/safety-b&feed=atom&action=historyTeam:SDU-Denmark/safety-b - Revision history2024-03-29T09:34:51ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/safety-b&diff=192631&oldid=prevToand: /* Photosensor (Part K343003) */2010-10-27T19:01:39Z<p><span class="autocomment">Photosensor (Part K343003)</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align: justify;">This BioBrick will not increase its hosts ability to survive in storage conditions, to be aerosoled, to be vaporized or create spores. None of its proteins regulate or affect the immune system or are pathogenic towards humans and animals.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align: justify;">This BioBrick will not increase its hosts ability to survive in storage conditions, to be aerosoled, to be vaporized or create spores. None of its proteins regulate or affect the immune system or are pathogenic towards humans and animals.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===References===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===References===</div></td></tr>
</table>Toandhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/safety-b&diff=166630&oldid=prevMarieBrønsNørgaard: /* Project safety */2010-10-26T21:12:42Z<p><span class="autocomment">Project safety</span></p>
<a href="http://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/safety-b&diff=166630&oldid=158307">Show changes</a>MarieBrønsNørgaardhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/safety-b&diff=158307&oldid=prevSøren: /* Risk-assessment for Individual Parts */2010-10-26T11:42:11Z<p><span class="autocomment">Risk-assessment for Individual Parts</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Methode===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Methode===</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="text-align: justify;">We have used our risk-assessment <del class="diffchange diffchange-inline">paper </del>to describe the safety of each of our contributed parts. The genes were [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLASTed] to find their function and known homologs. This function was then reasearched (mainly via [http://www.ncbi.nlm.nih.gov/PubMed PubMed]), described and considered according to the questions asked in the risk-assessment paper.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="text-align: justify;">We have used our <ins class="diffchange diffchange-inline">[https://2010.igem.org/Team:SDU-Denmark/safety-d#Procedure </ins>risk-assessment <ins class="diffchange diffchange-inline">guidelines] </ins>to describe the safety of each of our contributed parts. The genes were [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLASTed] to find their function and known homologs. This function was then reasearched (mainly via [http://www.ncbi.nlm.nih.gov/PubMed PubMed]), described and considered according to the questions asked in the risk-assessment paper.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K343001 Monooxygenase (Part K343001)]===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K343001 Monooxygenase (Part K343001)]===</div></td></tr>
</table>Sørenhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/safety-b&diff=158296&oldid=prevSøren: /* References */2010-10-26T11:40:57Z<p><span class="autocomment">References</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===References===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===References===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="text-align: justify;"># Spiegl N, Didichenko S, McCaffery P, Langen H, Dahinden CA. [http://www.ncbi.nlm.nih.gov/pubmed Human basophils activated by mast cell-derived IL-3 express retinaldehyde dehydrogenase-II and produce the immunoregulatory mediator retinoic acid]. Blood. 2008 Nov 1;112(9):3762-71.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="text-align: justify;"></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Spiegl N, Didichenko S, McCaffery P, Langen H, Dahinden CA. [http://www.ncbi.nlm.nih.gov/pubmed Human basophils activated by mast cell-derived IL-3 express retinaldehyde dehydrogenase-II and produce the immunoregulatory mediator retinoic acid]. Blood. 2008 Nov 1;112(9):3762-71.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Russell RM. [http://www.ncbi.nlm.nih.gov/pubmed/10731492 The vitamin A spectrum: from deficiency to toxicity]. American Journal of Clinical Nutrition, Vol. 71, No. 4, 878-884, April 2000.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Russell RM. [http://www.ncbi.nlm.nih.gov/pubmed/10731492 The vitamin A spectrum: from deficiency to toxicity]. American Journal of Clinical Nutrition, Vol. 71, No. 4, 878-884, April 2000.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Pasquali D, Thaller C, Eichele G. [http://www.ncbi.nlm.nih.gov/pubmed/8964849 Abnormal level of retinoic acid in prostate cancer tissues]. J Clin Endocrinol Metab. 1996 Jun;81(6):2186-91. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Pasquali D, Thaller C, Eichele G. [http://www.ncbi.nlm.nih.gov/pubmed/8964849 Abnormal level of retinoic acid in prostate cancer tissues]. J Clin Endocrinol Metab. 1996 Jun;81(6):2186-91. </div></td></tr>
</table>Sørenhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/safety-b&diff=158291&oldid=prevSøren: /* Project safety */2010-10-26T11:40:34Z<p><span class="autocomment">Project safety</span></p>
<a href="http://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/safety-b&diff=158291&oldid=158261">Show changes</a>Sørenhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/safety-b&diff=158261&oldid=prevSøren: /* References */2010-10-26T11:37:45Z<p><span class="autocomment">References</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Jung KH, Spudich EN, Trivedi VD, Spudich JL. [http://www.ncbi.nlm.nih.gov/pubmed An archaeal photosignal-transducing module mediates phototaxis in Escherichia coli]. J. Bacteriol. 2001 Nov;183(21):6365-6371. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Jung KH, Spudich EN, Trivedi VD, Spudich JL. [http://www.ncbi.nlm.nih.gov/pubmed An archaeal photosignal-transducing module mediates phototaxis in Escherichia coli]. J. Bacteriol. 2001 Nov;183(21):6365-6371. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Mennes N, Klare JP, Chizhov I, Seidel R, Schlesinger R, Engelhard M. [http://www.ncbi.nlm.nih.gov/pubmed Expression of the halobacterial transducer protein HtrII from Natronomonas pharaonis in Escherichia coli.] FEBS Lett. 2007 Apr 3;581(7):1487-1494. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Mennes N, Klare JP, Chizhov I, Seidel R, Schlesinger R, Engelhard M. [http://www.ncbi.nlm.nih.gov/pubmed Expression of the halobacterial transducer protein HtrII from Natronomonas pharaonis in Escherichia coli.] FEBS Lett. 2007 Apr 3;581(7):1487-1494. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Oxley APA, Lanfranconi MP, Würdemann D, Ott S, Schreiber S, McGenity TJ, et al. [http://www.ncbi.nlm.nih.gov/pubmed Halophilic archaea in the human intestinal mucosa]. Environ Microbiol [Internet]. 2010 Apr 23 [cited 2010 Oct 26]<del class="diffchange diffchange-inline">;Available from: http://www</del>.<del class="diffchange diffchange-inline">ncbi.nlm.nih.gov/pubmed/20438582</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Oxley APA, Lanfranconi MP, Würdemann D, Ott S, Schreiber S, McGenity TJ, et al. [http://www.ncbi.nlm.nih.gov/pubmed Halophilic archaea in the human intestinal mucosa]. Environ Microbiol [Internet]. 2010 Apr 23 [cited 2010 Oct 26].</div></td></tr>
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</table>Sørenhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/safety-b&diff=158251&oldid=prevSøren: /* References */2010-10-26T11:37:19Z<p><span class="autocomment">References</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===References===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===References===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Spiegl N, Didichenko S, McCaffery P, Langen H, Dahinden CA. Human basophils activated by mast cell-derived IL-3 express retinaldehyde dehydrogenase-II and produce the immunoregulatory mediator retinoic acid. Blood. 2008 Nov 1;112(9):3762-71.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Spiegl N, Didichenko S, McCaffery P, Langen H, Dahinden CA. <ins class="diffchange diffchange-inline">[http://www.ncbi.nlm.nih.gov/pubmed </ins>Human basophils activated by mast cell-derived IL-3 express retinaldehyde dehydrogenase-II and produce the immunoregulatory mediator retinoic acid<ins class="diffchange diffchange-inline">]</ins>. Blood. 2008 Nov 1;112(9):3762-71.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Russell RM. The vitamin A spectrum: from deficiency to toxicity. American Journal of Clinical Nutrition, Vol. 71, No. 4, 878-884, April 2000.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Russell RM. <ins class="diffchange diffchange-inline">[http://www.ncbi.nlm.nih.gov/pubmed/10731492 </ins>The vitamin A spectrum: from deficiency to toxicity<ins class="diffchange diffchange-inline">]</ins>. American Journal of Clinical Nutrition, Vol. 71, No. 4, 878-884, April 2000.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Pasquali D, Thaller C, Eichele G. Abnormal level of retinoic acid in prostate cancer tissues. J Clin Endocrinol Metab. 1996 Jun;81(6):2186-91. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Pasquali D, Thaller C, Eichele G. <ins class="diffchange diffchange-inline">[http://www.ncbi.nlm.nih.gov/pubmed/8964849 </ins>Abnormal level of retinoic acid in prostate cancer tissues<ins class="diffchange diffchange-inline">]</ins>. J Clin Endocrinol Metab. 1996 Jun;81(6):2186-91. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Bryant DA, Frigaard N. Prokaryotic photosynthesis and phototrophy illuminated. Trends Microbiol. 2006 Nov;14(11):488-496. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Bryant DA, Frigaard N. <ins class="diffchange diffchange-inline">[http://www.ncbi.nlm.nih.gov/pubmed </ins>Prokaryotic photosynthesis and phototrophy illuminated<ins class="diffchange diffchange-inline">]</ins>. Trends Microbiol. 2006 Nov;14(11):488-496. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Young GM, Badger JL, Miller VL. Motility is required to initiate host cell invasion by Yersinia enterocolitica. Infect. Immun. 2000 Jul;68(7):4323-4326. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Young GM, Badger JL, Miller VL. <ins class="diffchange diffchange-inline">[http://www.ncbi.nlm.nih.gov/pubmed </ins>Motility is required to initiate host cell invasion by Yersinia enterocolitica<ins class="diffchange diffchange-inline">]</ins>. Infect. Immun. 2000 Jul;68(7):4323-4326. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Gauger EJ, Leatham MP, Mercado-Lubo R, Laux DC, Conway T, Cohen PS. Role of Motility and the flhDC Operon in Escherichia coli MG1655 Colonization of the Mouse Intestine. Infect. Immun. 2007 Jul 1;75(7):3315-3324. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Gauger EJ, Leatham MP, Mercado-Lubo R, Laux DC, Conway T, Cohen PS. <ins class="diffchange diffchange-inline">[http://www.ncbi.nlm.nih.gov/pubmed </ins>Role of Motility and the flhDC Operon in Escherichia coli MG1655 Colonization of the Mouse Intestine<ins class="diffchange diffchange-inline">]</ins>. Infect. Immun. 2007 Jul 1;75(7):3315-3324. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Jung KH, Spudich EN, Trivedi VD, Spudich JL. An archaeal photosignal-transducing module mediates phototaxis in Escherichia coli. J. Bacteriol. 2001 Nov;183(21):6365-6371. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Jung KH, Spudich EN, Trivedi VD, Spudich JL. <ins class="diffchange diffchange-inline">[http://www.ncbi.nlm.nih.gov/pubmed </ins>An archaeal photosignal-transducing module mediates phototaxis in Escherichia coli<ins class="diffchange diffchange-inline">]</ins>. J. Bacteriol. 2001 Nov;183(21):6365-6371. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Mennes N, Klare JP, Chizhov I, Seidel R, Schlesinger R, Engelhard M. Expression of the halobacterial transducer protein HtrII from Natronomonas pharaonis in Escherichia coli. FEBS Lett. 2007 Apr 3;581(7):1487-1494. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Mennes N, Klare JP, Chizhov I, Seidel R, Schlesinger R, Engelhard M. <ins class="diffchange diffchange-inline">[http://www.ncbi.nlm.nih.gov/pubmed </ins>Expression of the halobacterial transducer protein HtrII from Natronomonas pharaonis in Escherichia coli.<ins class="diffchange diffchange-inline">] </ins>FEBS Lett. 2007 Apr 3;581(7):1487-1494. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># Oxley APA, Lanfranconi MP, Würdemann D, Ott S, Schreiber S, McGenity TJ, et al. Halophilic archaea in the human intestinal mucosa. Environ Microbiol [Internet]. 2010 Apr 23 [cited 2010 Oct 26];Available from: http://www.ncbi.nlm.nih.gov/pubmed/20438582</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># Oxley APA, Lanfranconi MP, Würdemann D, Ott S, Schreiber S, McGenity TJ, et al. <ins class="diffchange diffchange-inline">[http://www.ncbi.nlm.nih.gov/pubmed </ins>Halophilic archaea in the human intestinal mucosa<ins class="diffchange diffchange-inline">]</ins>. Environ Microbiol [Internet]. 2010 Apr 23 [cited 2010 Oct 26];Available from: http://www.ncbi.nlm.nih.gov/pubmed/20438582</div></td></tr>
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</table>Sørenhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/safety-b&diff=158165&oldid=prevSøren: /* Risk-assessment for Individual Parts */2010-10-26T11:30:41Z<p><span class="autocomment">Risk-assessment for Individual Parts</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There is not reason to believe this biobrick could be used for malign uses; it does not increase the hosts ability to vaporize, create spores, regulate the immunesystem or should be pathogenic. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There is not reason to believe this biobrick could be used for malign uses; it does not increase the hosts ability to vaporize, create spores, regulate the immunesystem or should be pathogenic. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">====Construct notes====</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''What is the origin of the genetic material used? What does the the genetic materiale do in this origin? Are there uncertainty about the genetical materials function? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The gene was cloned from Drosophila melanogaster cDNA. The normal function of the gene is to create beta-caroten monooxygenase as outlined above. The function of this gene is well characterized in the literature and there are little reason to suspect it should function otherwise. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''What modification were done on the genetic materiale before insertion? If anything was modified, what function do you hope to achieve? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">No changes were made to the DNA before inserting it into E. coli. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''What vector did you use? Which antibiotic resistance were involved? Which protocol was used to insert the vector? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The gene was inserted into two plasmid backbones, both containing chloramphenicol resistance. Both plasmids are specially made for BioBrick use and as such tested and safe. The plasmid was introduced into E. coli via chemical transformation. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''What is the stability of the insert with respect to genetic traits? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">We have not yet tested the stability of the organism after insertion of our BioBrick. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''How easily can the insert transfer to other bacteria or lifeforms? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">We have not tested the vectors ability to transfer the BioBrick to other bacteria. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''Where there safer alternatives to achieve this function? Where there safer alternatives to the host organism and vector used? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">We considered the gene, the strains of E. coli and used plasmids as safe. Cell-free systems might have been used, but these have yet to gain the same function as real bacteria.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K343000 Hyperflagellation (Part K343000)]===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K343000 Hyperflagellation (Part K343000)]===</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 109:</td>
<td colspan="2" class="diff-lineno">Line 84:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There is no reason to believe this BioBrick could be used for malign uses; it does not increase the host’s ability to vaporize, create spores or ability to survive under storage conditions. The fact that this BioBrick will likely increase the immune systems response to hosts carrying it makes it a bad candidate for malign use.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There is no reason to believe this BioBrick could be used for malign uses; it does not increase the host’s ability to vaporize, create spores or ability to survive under storage conditions. The fact that this BioBrick will likely increase the immune systems response to hosts carrying it makes it a bad candidate for malign use.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">====Construct notes====</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''What is the origin of the genetic material used? What does the the genetic materiale do in this origin? Are there uncertainty about the genetical materials function? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">This BioBrick is a modified gene from ''E. coli''. Its function is well known and described above. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''What modification were done on the genetic materiale before insertion? If anything was modified, what function do you hope to achieve? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">To make the part conform to the iGEM assembly standards we introduced a silent mutation, in an unintended restriction site, at BP 822 from t to c, so that Pst1 would not cleave the DNA there. We do not believe this makes the gene more pathogenic.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''What vector did you use? Which antibiotic resistance were involved? Which protocol was used to insert the vector?''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The gene was inserted into two plasmid backbones, both containing chloramphenicol resistance. Both plasmids are specially made for BioBrick use and as such tested and safe. The plasmid was introduced into E. coli via chemical transformation.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''What is the stability of the insert with respect to genetic traits? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">We have not yet tested the stability of the organism after insertion of our BioBrick. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''How easily can the insert transfer to other bacteria or lifeforms? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">We have not tested the vectors ability to transfer the BioBrick to other bacteria. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''Where there safer alternatives to achieve this function? Where there safer alternatives to the host organism and vector used? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">We considered the gene, the strains of E. coli and used plasmids as safe.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''Is your construct watermarked?''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">No.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K343003 Photosensor (Part K343003)]===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K343003 Photosensor (Part K343003)]===</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 159:</td>
<td colspan="2" class="diff-lineno">Line 107:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This BioBrick will not increase it’s hosts ability to survive in storage conditions, to be arosoled, to be vaporized or create spores. None of its proteins regulate or affect the immune system or are pathogenic towards humans and animals.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This BioBrick will not increase it’s hosts ability to survive in storage conditions, to be arosoled, to be vaporized or create spores. None of its proteins regulate or affect the immune system or are pathogenic towards humans and animals.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">====Construct notes====</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''What is the origin of the genetic material used? What does the the genetic materiale do in this origin? Are there uncertainty about the genetical materials function?''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The NpSopII part is from haloarchaea, the NpHtrII is from E. coli and has homologs in salmonella. The function is fairly well described and when BLASTing for each part, no homologs to pathogenic genes came up. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''What modification were done on the genetic materiale before insertion? If anything was modified, what function do you hope to achieve? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The BioBrick consists of different parts that were ligated together. Other then that, no modifications were done.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''What vector did you use? Which antibiotic resistance were involved? Which protocol was used to insert the vector? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The gene was inserted into two plasmid backbones, both containing chloramphenicol resistance. Both plasmids are specially made for BioBrick use and as such tested and safe. The plasmid was introduced into E. coli via chemical transformation.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''What is the stability of the insert with respect to genetic traits?''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The stability of the plasmids we used seems safe; almost none of our bacteria throw the plasmid.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''How easily can the insert transfer to other bacteria or lifeforms? ''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">We have not tested the vectors ability to transfer the BioBrick to other bacteria. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''Where there safer alternatives to achieve this function? Where there safer alternatives to the host organism and vector used?''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">We considered the gene, the strains of E. coli and used plasmids as safe.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">''Is your construct watermarked?''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">No.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===References===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===References===</div></td></tr>
</table>Sørenhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/safety-b&diff=158145&oldid=prevSøren: /* Possible malign use */2010-10-26T11:29:00Z<p><span class="autocomment">Possible malign use</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There is not reason to believe this biobrick could be used for malign uses; it does not increase the hosts ability to vaporize, create spores, regulate the immunesystem or should be pathogenic. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There is not reason to believe this biobrick could be used for malign uses; it does not increase the hosts ability to vaporize, create spores, regulate the immunesystem or should be pathogenic. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Construct notes</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">====</ins>Construct notes<ins class="diffchange diffchange-inline">====</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''What is the origin of the genetic material used? What does the the genetic materiale do in this origin? Are there uncertainty about the genetical materials function? ''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''What is the origin of the genetic material used? What does the the genetic materiale do in this origin? Are there uncertainty about the genetical materials function? ''</div></td></tr>
</table>Sørenhttp://2010.igem.org/wiki/index.php?title=Team:SDU-Denmark/safety-b&diff=158110&oldid=prevSøren: /* Risk-assessment for Individual Parts */2010-10-26T11:26:49Z<p><span class="autocomment">Risk-assessment for Individual Parts</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The BioBrick’s product is not in itself toxic, but we do not recommend using this BioBrick for any type of system in humans or animals for the following reasons: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The BioBrick’s product is not in itself toxic, but we do not recommend using this BioBrick for any type of system in humans or animals for the following reasons: </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Retinoic acid, which retinal can degrade into, can affect gene expression and function of almost any cell, including cells of the immune system; it also plays a fundamental role in cellular functions by activating nuclear receptors (1). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Retinoic acid, which retinal can degrade into, can affect gene expression and function of almost any cell, including cells of the immune system; it also plays a fundamental role in cellular functions by activating nuclear receptors (<ins class="diffchange diffchange-inline">[https://2010.igem.org/Team:SDU-Denmark/safety-b#References </ins>1<ins class="diffchange diffchange-inline">]</ins>). </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Vitamin A toxicity can lead to hepatic congestion and fibrosis (2). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Vitamin A toxicity can lead to hepatic congestion and fibrosis (<ins class="diffchange diffchange-inline">[https://2010.igem.org/Team:SDU-Denmark/safety-b#References </ins>2<ins class="diffchange diffchange-inline">]</ins>). </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Vitamin A and its derivatives have been implicated as chemopreventive and differentiating agents in a variety of cancers (3). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Vitamin A and its derivatives have been implicated as chemopreventive and differentiating agents in a variety of cancers (<ins class="diffchange diffchange-inline">[https://2010.igem.org/Team:SDU-Denmark/safety-b#References </ins>3<ins class="diffchange diffchange-inline">]</ins>). </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>These effects are not directly associated with the enzyme itself, but have been observed in humans. It is highly unlikely that high enough doses can be reached with this biobrick. Please see references for more information about the diseases. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>These effects are not directly associated with the enzyme itself, but have been observed in humans. It is highly unlikely that high enough doses can be reached with this biobrick. Please see references for more information about the diseases. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The biobrick should not increase its host’s ability to spread, survive outside the laboratory, and will most likely decrease its ability to replicate.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The biobrick should not increase its host’s ability to spread, survive outside the laboratory, and will most likely decrease its ability to replicate.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Beta-caroten monooxygenase is found in a wide variety of different bacteria, insects and animals (4). As such we would be cautious as to letting a system containing this BioBrick into the wild, since it's function might conflict with existing systems. On the other hand one might argue that since it's function is already available in nature, the function is widely available. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Beta-caroten monooxygenase is found in a wide variety of different bacteria, insects and animals (<ins class="diffchange diffchange-inline">[https://2010.igem.org/Team:SDU-Denmark/safety-b#References </ins>4<ins class="diffchange diffchange-inline">]</ins>). As such we would be cautious as to letting a system containing this BioBrick into the wild, since it's function might conflict with existing systems. On the other hand one might argue that since it's function is already available in nature, the function is widely available. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The product of the BioBrick, retinal, also plays an important function in nature and animals. For this reason we fell that the BioBrick could be used in controlled settings, but not in the wild. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The product of the BioBrick, retinal, also plays an important function in nature and animals. For this reason we fell that the BioBrick could be used in controlled settings, but not in the wild. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Potential pathogenicity====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Potential pathogenicity====</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This biobrick increases the potential of its host to move. Increased motility has been associated the bacteria’s ability to invade humans (5); on the other hand it has also been shown that bacteria that loss the function of the FlhDC operon, are considerable better at colonizing the intestine (6), and so an increased expression might decrease a hosts ability to colonize and invade humans. It is impossible to ensure, that this plasmid is not transferred to pathogenic bacteria since the FlhDC operons is used in a wide array of other bacteria that are known to be pathogenic.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This biobrick increases the potential of its host to move. Increased motility has been associated the bacteria’s ability to invade humans (<ins class="diffchange diffchange-inline">[https://2010.igem.org/Team:SDU-Denmark/safety-b#References </ins>5<ins class="diffchange diffchange-inline">]</ins>); on the other hand it has also been shown that bacteria that loss the function of the FlhDC operon, are considerable better at colonizing the intestine (<ins class="diffchange diffchange-inline">[https://2010.igem.org/Team:SDU-Denmark/safety-b#References </ins>6<ins class="diffchange diffchange-inline">]</ins>), and so an increased expression might decrease a hosts ability to colonize and invade humans. It is impossible to ensure, that this plasmid is not transferred to pathogenic bacteria since the FlhDC operons is used in a wide array of other bacteria that are known to be pathogenic.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A number of effector cells in the human immune system react specifically to bacteria’s flagella, and so a hyperflagellated host will most likely induce a stronger immune response. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A number of effector cells in the human immune system react specifically to bacteria’s flagella, and so a hyperflagellated host will most likely induce a stronger immune response. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Potential pathogenicity====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Potential pathogenicity====</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This BioBrick consists of three different parts: The first 224 amino acid residues come from the NpSopII gene from halobacteria, encoding a blue-light photon receptor with 15 residues removed at the C-terminal. The following 9 amino acids are a linker. The last part is HtrII fused with Tsr from E. Coli. The complex' first 125 amino acid residues come from HtrII and the remaining 279 from Tsr (7). NpHtrII is thought to function in signal transduction and activation of microbial signalling cascades (8). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This BioBrick consists of three different parts: The first 224 amino acid residues come from the NpSopII gene from halobacteria, encoding a blue-light photon receptor with 15 residues removed at the C-terminal. The following 9 amino acids are a linker. The last part is HtrII fused with Tsr from E. Coli. The complex' first 125 amino acid residues come from HtrII and the remaining 279 from Tsr (<ins class="diffchange diffchange-inline">[https://2010.igem.org/Team:SDU-Denmark/safety-b#References </ins>7<ins class="diffchange diffchange-inline">]</ins>). NpHtrII is thought to function in signal transduction and activation of microbial signalling cascades (<ins class="diffchange diffchange-inline">[https://2010.igem.org/Team:SDU-Denmark/safety-b#References </ins>8<ins class="diffchange diffchange-inline">]</ins>). </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>A single article has been written about haloarchaea in humans indicating that these played a role in patients with inflammatory bowl disease (9), but there is no evidence that the genes or near homologs, this BioBrick is made from, are involved in any disease process, toxic products or invasion properties. They do not regulate the immune system in any way.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>A single article has been written about haloarchaea in humans indicating that these played a role in patients with inflammatory bowl disease (<ins class="diffchange diffchange-inline">[https://2010.igem.org/Team:SDU-Denmark/safety-b#References </ins>9<ins class="diffchange diffchange-inline">]</ins>), but there is no evidence that the genes or near homologs, this BioBrick is made from, are involved in any disease process, toxic products or invasion properties. They do not regulate the immune system in any way.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Environmental impact====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Environmental impact====</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Is your construct watermarked?''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Is your construct watermarked?''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>No.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>No.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===References===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===References===</div></td></tr>
</table>Søren