Team:SDU-Denmark/project-p

From 2010.igem.org

(Difference between revisions)
(Flagella staining)
(HPLC determination of beta-carotene and retinal production)
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MG1655 containing PSB1A2 with K274210 plus PSB1C3 with K343005 both under a constitutively active promotor<br>  
MG1655 containing PSB1A2 with K274210 plus PSB1C3 with K343005 both under a constitutively active promotor<br>  
The measurements were preformed on cells after 20 hours of growth. The resulting graphs is presented beneath the text.<br>
The measurements were preformed on cells after 20 hours of growth. The resulting graphs is presented beneath the text.<br>
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[[Image:Team SDU-Denmark-HPLC samples2.png|thumb|left|300px|HPLC analysis of Beta-carotene. Peaks are clearly pressent. Measurements were made after 20 hours of growth]]
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[[Image:Team SDU-Denmark-HPLC samples2.png|thumb|left|300px|HPLC analysis of samples from the 19/10-10 Beta-carotene Peaks are clearly pressent, but no retinal peaks is seen. Measurements were made after 20 hours of growth]]
[[Image:Team SDU-Denmark-HPLC samples1.png|thumb|center|300px|HPLC analysis of retinal. Peaks are clearly pressent. Measurements were made after 20 hours of growth]]<br>
[[Image:Team SDU-Denmark-HPLC samples1.png|thumb|center|300px|HPLC analysis of retinal. Peaks are clearly pressent. Measurements were made after 20 hours of growth]]<br>
When looking at the retinal and beta-carotene standards analysed on the HPLC, peaks for both chemical are clearly present. The retinal retention time is 3,480-3,490 minutes and the beta-carotene retention time is 23,300-23,600 minutes.<br>     
When looking at the retinal and beta-carotene standards analysed on the HPLC, peaks for both chemical are clearly present. The retinal retention time is 3,480-3,490 minutes and the beta-carotene retention time is 23,300-23,600 minutes.<br>     

Revision as of 17:09, 27 October 2010