Team:SDU-Denmark/K343004

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(Phase contrast of ON cultures)
(Scanning Electron microscopy)
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=== Scanning Electron microscopy ===
=== Scanning Electron microscopy ===
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After the failed staning of the flagella we tried to visualize the flagella with scanning electron microscopy (SEM). The bacteria were grown ON in liquid cultures (5 ml LB-media). The bacteria were diluted to approximately 10^6 cells pr 10 µl solution. At that time we did not have our K343004 composite part so therefore we only tried SEM with the negative control strain DH5alpa and wild type MG1655. We did this as a preliminary work so we would be ready to do microscopy on the MG1655 cells containing the composite part when it was ready. Unfortunately the limited resolution and magnitude of the SEM at our disposal made it practically impossible to visualize any flagella in the microscope. Thus if this approach is to be used for characterization, a SEM of higher magnitude and resolution is required.<br>  
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After the failed staning of the flagella we tried to visualize the flagella with scanning electron microscopy (SEM). The bacteria were grown ON in liquid cultures (5 ml LB-media). The bacteria were diluted to approximately 10^6 cells pr 10 µl solution. At that time we did not have our K343004 composite part so therefore we only tried SEM with the negative control strain DH5alpa and wild type MG1655. We did this as a preliminary work so we would be ready to do microscopy on the MG1655 cells containing the composite part when it was ready. Unfortunately the limited resolution and magnitude of the SEM at our disposal made it practically impossible to visualize any flagella in the microscope. Thus if this approach is to be used for characterization, a SEM of higher magnitude and resolution is required. Our plan was instead to use a transmission electron microscope but unfortunately it was been unfunctional for that last week. <br>  
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The pictures below are SEM pictures the negative control strain DH5alpha and wild type MG1655.<br>br>
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The pictures below are SEM pictures the negative control strain DH5alpha and wild type MG1655.<br> <br>
[[Image:Team SDU-Denmark_DH5alpha_MAG_5.0_kx_and_EHT_7.5_kV.JPG|thumb|left|300px|SEM picture of DH5alpa a negative control that should not express flagella]][[Image:Team_SDU-Denmark_Mg1655_MAG5kx_and_EHT_7.5kV.JPG|thumb|center|300px|SEM picture of the ''E. coli'' strain MG1655 wild type this strain of bacteria should express approximately 4 flagella per cell.However, no flagella are visable]]
[[Image:Team SDU-Denmark_DH5alpha_MAG_5.0_kx_and_EHT_7.5_kV.JPG|thumb|left|300px|SEM picture of DH5alpa a negative control that should not express flagella]][[Image:Team_SDU-Denmark_Mg1655_MAG5kx_and_EHT_7.5kV.JPG|thumb|center|300px|SEM picture of the ''E. coli'' strain MG1655 wild type this strain of bacteria should express approximately 4 flagella per cell.However, no flagella are visable]]

Revision as of 01:14, 28 October 2010