Team:Queens-Canada/parts

From 2010.igem.org

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'''Note:''' The following list is tentative, and better reflects our goals rather than our current accomplishments. We are still in the process of isolating and characterising these components.
=General=
=General=
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|Enhanced Cyan Fluorescent Protein. From Chin-Sang plasmid.
|Enhanced Cyan Fluorescent Protein. From Chin-Sang plasmid.
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==Backbones==
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* Since it is possible to microinject plasmids into ''C. elegans'', we're working on a combination backbone that will produce GFP in both ''E. coli'' and ''C. elegans'', as well as providing antibiotic selection markers for ''E. coli'', and the cutsites from Standard 10.
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* Another project we're pursuing is the possible usage of double-stranded RNA as a liaison for DNA import. If this works, we'll have hybrid strand backbones for that method of transformation as well.
=Optogenetics (R.''C. elegans'')=
=Optogenetics (R.''C. elegans'')=

Revision as of 17:20, 23 June 2010

Part Structure

Show/hide parts as entered in the [http://partsregistry.org Registry].

Note: The following list is tentative, and better reflects our goals rather than our current accomplishments. We are still in the process of isolating and characterising these components.

General

Promoters

Name Description Strength
sip-1 Heat shock
rab-7 Constitutive high
mec-7 Mechanosensory neurons
hsp-3 Heat shock
gpd-2 Constitutive very high
unc-47 GABA-using (gabergic) neurons

Coding Sequences

Name Description
GFP Enhanced Green Fluorescent Protein. From biobrick BBa_I714891. Proof of concept to see if genes can be imported from bacteria.
eGFP Enhanced Green Fluorescent Protein. From Chin-Sang plasmid pIC691.
mCherry Red Fluorescent Protein. From Chin-Sang plasmid.
YFP Yellow Fluorescent Protein. From Chin-Sang plasmid.
eCFP Enhanced Cyan Fluorescent Protein. From Chin-Sang plasmid.

Backbones

  • Since it is possible to microinject plasmids into C. elegans, we're working on a combination backbone that will produce GFP in both E. coli and C. elegans, as well as providing antibiotic selection markers for E. coli, and the cutsites from Standard 10.
  • Another project we're pursuing is the possible usage of double-stranded RNA as a liaison for DNA import. If this works, we'll have hybrid strand backbones for that method of transformation as well.

Optogenetics (R.C. elegans)

Promoters

Name Description Strength

Coding Squences

Name Description
ChR2 Channelrhodopsin-2. Permits entry of positive ions in response to 460 nm light. Can be used to trigger nerve cells.
NpHR Halorhodopsin. Permits entry of chloride ions in response to 580 nm light. Can be used to block nerve cells from firing.