Team:Queens-Canada/30 August 2010

From 2010.igem.org

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'''pSip-1'''::1C3,  '''pFlp-1'''::1C3, '''pOdr-10'''::1C3, '''mCherry'''::1C3
'''pSip-1'''::1C3,  '''pFlp-1'''::1C3, '''pOdr-10'''::1C3, '''mCherry'''::1C3
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{| !border="1"
+
{| border="1"
| EcoRI and SpeI
| EcoRI and SpeI
| XbaI and PstI
| XbaI and PstI
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'''pSip-1''', '''pStr-220''', '''pFlp-1''', '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1T3
'''pSip-1''', '''pStr-220''', '''pFlp-1''', '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1T3
-
{|
+
{| border="1"
| EcoRI and SpeI
| EcoRI and SpeI
| XbaI and PstI
| XbaI and PstI
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'''pSip-1''', '''pStr-220''', '''pFlp-1''', '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1T3
'''pSip-1''', '''pStr-220''', '''pFlp-1''', '''pOdr-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1T3
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Transformed ligation products from [[Team:Queens-Canada/30 August 2010|30/08/10]]
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Transformed ligation products from [[Team:Queens-Canada/30 August 2010|30/08/10]].
<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3>
<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3>
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'''pMec-7'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3
'''pMec-7'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3
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Transferred F1 transgenic worms to new plate
+
Transferred F1 transgenic worms to new plate.
{{:Team:Queens-Canada/foot}}
{{:Team:Queens-Canada/foot}}

Latest revision as of 02:08, 28 October 2010

August 30, 2010

Liquid Culture

Thai

pOsm-10::eCFP::unc-54 3'UTR::1A3, pSra-10::eCFP::unc-54 3'UTR::1A3

Digestion

Hao and Thai

pSip-1::1C3, pFlp-1::1C3, pOdr-10::1C3, mCherry::1C3

EcoRI and SpeI XbaI and PstI EcoRI and PstI
pStr-220::1C3 eCFP::unc-54 3'UTR::1T3 pSB1T3
mCherry::1C3 unc-54 3'UTR::1C3
pFlp-1::1C3
pSip-1::1C3
pOdr-10::1C3

Backbone treated with CIAP for 30 mins.

Ligation

Hao and Thai

pSip-1, pStr-220, pFlp-1, pOdr-10::eCFP::unc-54 3'UTR::1A3, ChR2::unc-54 3'UTR::1T3

EcoRI and SpeI XbaI and PstI EcoRI and PstI
pStr-1 eCFP::unc-54 3'UTR 1A3
pStr-220 eCFP::unc-54 3'UTR 1A3
pFlp-1 eCFP::unc-54 3'UTR 1A3
pOdr-10 eCFP::unc-54 3'UTR 1A3
mCherry unc-54 3'UTR 1T3

Backbone treated with CIAP for 30 mins.

Heat Shock

Hao

pSip-1, pStr-220, pFlp-1, pOdr-10::eCFP::unc-54 3'UTR::1A3, ChR2::unc-54 3'UTR::1T3

Transformed ligation products from 30/08/10.

Microinjection

Hao

pMec-7::eCFP::unc-54 3'UTR::1C3

Transferred F1 transgenic worms to new plate.