Team:Queens-Canada/21 June 2010

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<h2>June 21, 2010</h2>
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On this day...
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* We got the wiki up and going!
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* We ran a crazy gel and cut it up a whole bunch.
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<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3>
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Hao
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'''pSip-1'''::'''eGFP''', '''pRab-7'''::'''eGFP''', '''pMec-7'''::'''eGFP'''
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Used 0.3 g agarose in 40 mL TBE. Gel run for 40 mins at 100 V.
 +
 
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<h3>[[Team:Queens-Canada/protocols#Gel Extraction|Gel Extraction]]</h3>
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Yuli and Hao
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'''pSip-1'''::'''eGFP''', '''pRab-7'''::'''eGFP''', '''pMec-7'''::'''eGFP'''
 +
 
 +
Products stored at -20 °C.
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<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3>
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Hao and Yuli
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Practiced injections with water.
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{{:Team:Queens-Canada/foot}}

Latest revision as of 01:08, 28 October 2010


June 21, 2010

On this day...

  • We got the wiki up and going!
  • We ran a crazy gel and cut it up a whole bunch.

Gel Electrophoresis

Hao

pSip-1::eGFP, pRab-7::eGFP, pMec-7::eGFP

Used 0.3 g agarose in 40 mL TBE. Gel run for 40 mins at 100 V.

Gel Extraction

Yuli and Hao

pSip-1::eGFP, pRab-7::eGFP, pMec-7::eGFP

Products stored at -20 °C.

Microinjection

Hao and Yuli

Practiced injections with water.