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Team:Queens-Canada/14 October 2010
From 2010.igem.org
(New page: <h2>October 14, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Heat Shock</h3> Steve '''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3 from [[Team...) |
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<h2>October 14, 2010</h2> | <h2>October 14, 2010</h2> | ||
{{:Team:Queens-Canada/head}} | {{:Team:Queens-Canada/head}} | ||
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Steve | Steve | ||
| - | '''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3 from [[Team:Queens-Canada/ | + | '''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3 from [[Team:Queens-Canada/7 October 2010|07/10/10]] |
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3> | <h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3> | ||
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<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | <h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | ||
Steve | Steve | ||
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Ligation products from [[Team:Queens-Canada/14 October 2010|14/10/10]] | Ligation products from [[Team:Queens-Canada/14 October 2010|14/10/10]] | ||
Used 0.3 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V. | Used 0.3 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V. | ||
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| + | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
| + | Hao | ||
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| + | '''pStr-220'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3 | ||
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| + | Co-injected construct with pRF4 marker. | ||
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| + | '''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pHsp-3'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pFlp-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3 | ||
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| + | Examined transgenic worms under confocal microscope; no fluorescence detected in target neuron. | ||
{{:Team:Queens-Canada/foot}} | {{:Team:Queens-Canada/foot}} | ||
Latest revision as of 03:44, 28 October 2010
October 14, 2010
Heat Shock
Steve
pStr-1::eCFP::unc-54 3'UTR::1C3 from 07/10/10
Liquid Culture
Steve
pFlp-1::eCFP::unc-54 3'UTR::1C3, pSra-10::eCFP::unc-54 3'UTR::1C3, ChR2::eYFP::unc-54 3'UTR::1C3, pHsp-3::eCFP::unc-54 3'UTR::1C3, pOsm-10::eCFP::unc-54 3'UTR::1C3, pOdr-1::eCFP::unc-54 3'UTR::1C3, eYFP::unc-54 3'UTR::1C3, mCherry::unc-54 3'UTR::1C3
Digestion-Ligation
Steve
pGpd-2, pOdr-10, pStr-220, pOdr-10, pSip-1, eCFP::unc-54 3'UTR::1C3
Gel Electrophoresis
Steve
Ligation products from 14/10/10
Used 0.3 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.
Microinjection
Hao
pStr-220::eCFP::unc-54 3'UTR::1A3
Co-injected construct with pRF4 marker.
pStr-1::eCFP::unc-54 3'UTR::1A3, pHsp-3::eCFP::unc-54 3'UTR::1A3, pFlp-1::eCFP::unc-54 3'UTR::1A3
Examined transgenic worms under confocal microscope; no fluorescence detected in target neuron.
