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Team:Purdue/Seed plating
From 2010.igem.org
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| Line 2: | Line 2: | ||
[[Seed sterilization link]] | [[Seed sterilization link]] | ||
| + | |||
[[Agar prep link]] | [[Agar prep link]] | ||
| Line 13: | Line 14: | ||
#plates | #plates | ||
#parafilm tape | #parafilm tape | ||
| + | #70% ethanol solution in spray bottle | ||
==Methods== | ==Methods== | ||
| - | # | + | #Sterilize items one by one with 70% ethanol spray, placing each item into sterile hood after being sprayed. |
| + | #Once all is in hood, remove aluminum foil wrap from agar flask. | ||
| + | #open first plate and | ||
#don't fill too much, use finger press method | #don't fill too much, use finger press method | ||
| + | |||
| + | Notes: | ||
| + | |||
| + | Keep head out of hood. Contamination from hair, mouth, nose, hands, etc. will infect culture plate with mold spores. | ||
Revision as of 15:10, 8 July 2010
Sterile Agar Plate Preparation and Seed Placement
Materials
- sterile seed in flask
- sterile agar media
- sterile hood environment
- P1000 pipeter
- blue pipeter tips
- plates
- parafilm tape
- 70% ethanol solution in spray bottle
Methods
- Sterilize items one by one with 70% ethanol spray, placing each item into sterile hood after being sprayed.
- Once all is in hood, remove aluminum foil wrap from agar flask.
- open first plate and
- don't fill too much, use finger press method
Notes:
Keep head out of hood. Contamination from hair, mouth, nose, hands, etc. will infect culture plate with mold spores.
