Team:Purdue/MS media preparation

From 2010.igem.org

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(Procedure)
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==Procedure==
==Procedure==
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Use container that is twice as large as solution volume (ex. for 1 L of media, use a 2 L flask). Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7 using 1 N NaOH or KOH solutions.  Finally, add agar.  There is no need to dissolve the agar prior to autoclaving.
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Use container that is twice as large as solution volume (ex. for 1 L of media, use a 2 L flask). Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7 using 1 N NaOH or KOH solutions.  Finally, add agar.  There is no need to dissolve the agar prior to autoclaving. Cover flask/container with double layer aluminum foil. Place in autoclave and select P6-Liquid-Long Cycle. Total time for autoclave is 60 min.
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==Autoclave Notes==
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Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min.
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Revision as of 14:46, 8 July 2010

For one liter of 0.5 MS:

  • 2.165 g MS salt
  • 15 g sucrose (1.5%)
  • 12 g agar (1.2%)
  • 1 N NaOH or KOH

Procedure

Use container that is twice as large as solution volume (ex. for 1 L of media, use a 2 L flask). Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7 using 1 N NaOH or KOH solutions. Finally, add agar. There is no need to dissolve the agar prior to autoclaving. Cover flask/container with double layer aluminum foil. Place in autoclave and select P6-Liquid-Long Cycle. Total time for autoclave is 60 min.