Team:Panama/8 October 2010
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(New page: Concentration Measurement of the purified PCR product 107ng/ul Proportional Calculation for repeating the cloning with pGEM-T Easy Vector System: Insert:Vector molar ratios Recommended ...) |
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Latest revision as of 19:15, 27 October 2010
October 8
Concentration Measurement of the purified PCR product:107ng/ul
Proportional Calculation for repeating the cloning with pGEM-T Easy Vector System:
Insert:Vector molar ratios Recommended ratios: 3:1, 1:3
Vector (3kb) = 50 ng/ul
ng insert= (ng vector x kb insert/kb vector) x insert:vector molar ratio
50 ng vector x 2.2 kb insert/3 kb vector x 3:1 = 110 ng insert
110 ng = 1.03 ul of insert
Today we repeated the cloning and the ligation
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