Team:Panama/4 October 2010

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Ligation of Reporter and Terminator (About two hours)

We used the same quantities that were used on September 23 2010

PCR (About 2.5 hours)

On October 1, we did the miniprep of the cloning product of the gene Rh1ab in the pGEM-T vector

Today, we will do a PCR of this to see whether the clones that have grown contain the gene Rh1ab

Photo

No bands appear

Possible reasons:

1) The PCR has had some sort of problem

2) The cloning has had some sort of problem

Possible solutions:

1) Repeat the PCR, changing the denaturizing temperature time to 8 minutes and in the cycling from 30 seconds to 1 minute. Besides that, we used two different miniprep samples from the cloning (maybe the colony that we picked doesn't have the insert)

Photo

May
M	T	W	T	F	S	S
					[http://2010.igem.org/wiki/index.php?title=Team:Panama/1_May_2010&action=edit 1]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/2_May_2010&action=edit 2]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/3_May_2010&action=edit 3]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/4_May_2010&action=edit 4]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/5_May_2010&action=edit 5]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/6_May_2010&action=edit 6]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/7_May_2010&action=edit 7]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/8_May_2010&action=edit 8]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/9_May_2010&action=edit 9]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/10_May_2010&action=edit 10]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/11_May_2010&action=edit 11]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/12_May_2010&action=edit 12]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/13_May_2010&action=edit 13]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/14_May_2010&action=edit 14]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/15_May_2010&action=edit 15]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/16_May_2010&action=edit 16]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/17_May_2010&action=edit 17]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/18_May_2010&action=edit 18]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/19_May_2010&action=edit 19]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/20_May_2010&action=edit 20]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/21_May_2010&action=edit 21]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/22_May_2010&action=edit 22]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/23_May_2010&action=edit 23]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/24_May_2010&action=edit 24]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/25_May_2010&action=edit 25]	[http://2010.igem.org/Team:Panama/26_May_2010 26]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/27_May_2010&action=edit 27]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/28_May_2010&action=edit 28]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/29_May_2010&action=edit 29]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/30_May_2010&action=edit 30]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/31_May_2010&action=edit 31]

June
M	T	W	T	F	S	S
	[http://2010.igem.org/wiki/index.php?title=Team:Panama/1_June_2010&action=edit 1]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/2_June_2010&action=edit 2]	[http://2010.igem.org/Team:Panama/3_June_2010 3]	[http://2010.igem.org/Team:Panama/4_June_2010 4]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/5_June_2010&action=edit 5]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/6_June_2010&action=edit 6]
[http://2010.igem.org/Team:Panama/7_June_2010 7]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/8_June_2010&action=edit 8]	[http://2010.igem.org/Team:Panama/9_June_2010 9]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/10_June_2010&action=edit 10]	[http://2010.igem.org/Team:Panama/11_June_2010 11]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/12_June_2010&action=edit 12]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/13_June_2010&action=edit 13]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/14_June_2010&action=edit 14]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/15_June_2010&action=edit 15]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/16_June_2010&action=edit 16]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/17_June_2010&action=edit 17]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/18_June_2010&action=edit 18]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/19_June_2010&action=edit 19]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/20_June_2010&action=edit 20]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/21_June_2010&action=edit 21]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/22_June_2010&action=edit 22]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/23_June_2010&action=edit 23]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/24_June_2010&action=edit 24]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/25_June_2010&action=edit 25]	[http://2010.igem.org/Team:Panama/26_June_2010 26]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/27_June_2010&action=edit 27]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/28_June_2010&action=edit 28]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/29_June_2010&action=edit 29]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/30_June_2010&action=edit 30]

July
M	T	W	T	F	S	S
			[http://2010.igem.org/wiki/index.php?title=Team:Panama/1_July_2010&action=edit 1]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/2_July_2010&action=edit 2]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/3_July_2010&action=edit 3]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/4_July_2010&action=edit 4]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/5_July_2010&action=edit 5]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/6_July_2010&action=edit 6]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/7_July_2010&action=edit 7]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/8_July_2010&action=edit 8]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/9_July_2010&action=edit 9]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/10_July_2010&action=edit 10]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/11_July_2010&action=edit 11]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/12_July_2010&action=edit 12]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/13_July_2010&action=edit 13]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/14_July_2010&action=edit 14]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/15_July_2010&action=edit 15]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/16_July_2010&action=edit 16]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/17_July_2010&action=edit 17]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/18_July_2010&action=edit 18]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/19_July_2010&action=edit 19]	[http://2010.igem.org/Team:Panama/20_July_2010 20]	[http://2010.igem.org/Team:Panama/21_July_2010 21]	[http://2010.igem.org/Team:Panama/22_July_2010 22]	[http://2010.igem.org/Team:Panama/23_July_2010 23]	[http://2010.igem.org/Team:Panama/24_July_2010 24]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/25_July_2010&action=edit 25]
[http://2010.igem.org/Team:Panama/26_July_2010 26]	[http://2010.igem.org/Team:Panama/27_July_2010 27]	[http://2010.igem.org/Team:Panama/28_July_2010 28]	[http://2010.igem.org/Team:Panama/29_July_2010 29]	[http://2010.igem.org/Team:Panama/30_July_2010 30]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/31_July_2010&action=edit 31]

August
M	T	W	T	F	S	S
						[http://2010.igem.org/wiki/index.php?title=Team:Panama/1_August_2010&action=edit 1]
[http://2010.igem.org/Team:Panama/2_August_2010 2]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/3_August_2010&action=edit 3]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/4_August_2010&action=edit 4]	[http://2010.igem.org/Team:Panama/5_August_2010 5]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/6_August_2010&action=edit 6]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/7_August_2010&action=edit 7]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/8_August_2010&action=edit 8]
[http://2010.igem.org/Team:Panama/9_August_2010 9]	[http://2010.igem.org/Team:Panama/10_August_2010 10]	[http://2010.igem.org/Team:Panama/11_August_2010 11]	[http://2010.igem.org/Team:Panama/12_August_2010 12]	[http://2010.igem.org/Team:Panama/13_August_2010 13]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/14_August_2010&action=edit 14]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/15_August_2010&action=edit 15]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/16_August_2010&action=edit 16]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/17_August_2010&action=edit 17]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/18_August_2010&action=edit 18]	[http://2010.igem.org/Team:Panama/19_August_2010 19]	[http://2010.igem.org/Team:Panama/20_August_2010 20]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/21_August_2010&action=edit 21]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/22_August_2010&action=edit 22]
[http://2010.igem.org/Team:Panama/23_August_2010 23]	[http://2010.igem.org/Team:Panama/24_August_2010 24]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/25_August_2010&action=edit 25]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/26_August_2010&action=edit 26]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/27_August_2010&action=edit 27]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/28_August_2010&action=edit 28]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/29_August_2010&action=edit 29]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/30_August_2010&action=edit 30]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/31_August_2010&action=edit 31]

September
M	T	W	T	F	S	S
		[http://2010.igem.org/wiki/index.php?title=Team:Panama/1_September_2010&action=edit 1]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/2_September_2010&action=edit 2]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/3_September_2010&action=edit 3]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/4_September_2010&action=edit 4]	[http://2010.igem.org/Team:Panama/5_September_2010 5]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/6_September_2010&action=edit 6]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/7_September_2010&action=edit 7]	[http://2010.igem.org/Team:Panama/8_September_2010 8]	[http://2010.igem.org/Team:Panama/9_September_2010 9]	[http://2010.igem.org/Team:Panama/10_September_2010 10]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/11_September_2010&action=edit 11]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/12_September_2010&action=edit 12]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/13_September_2010&action=edit 13]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/14_September_2010&action=edit 14]	[http://2010.igem.org/Team:Panama/15_September_2010 15]	[http://2010.igem.org/Team:Panama/16_September_2010 16]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/17_September_2010&action=edit 17]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/18_September_2010&action=edit 18]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/19_September_2010&action=edit 19]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/20_September_2010&action=edit 20]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/21_September_2010&action=edit 21]	[http://2010.igem.org/Team:Panama/22_September_2010 22]	[http://2010.igem.org/Team:Panama/23_September_2010 23]	[http://2010.igem.org/Team:Panama/24_September_2010 24]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/25_September_2010&action=edit 25]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/26_September_2010&action=edit 26]
[http://2010.igem.org/wiki/index.php?title=Team:Panama/27_September_2010&action=edit 27]	[http://2010.igem.org/Team:Panama/28_September_2010 28]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/29_September_2010&action=edit 29]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/30_September_2010&action=edit 30]

October
M	T	W	T	F	S	S
				[http://2010.igem.org/wiki/index.php?title=Team:Panama/1_October_2010&action=edit 1]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/2_October_2010&action=edit 2]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/3_October_2010&action=edit 3]
[http://2010.igem.org/Team:Panama/4_October_2010 4]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/5_October_2010&action=edit 5]	[http://2010.igem.org/Team:Panama/6_October_2010 6]	[http://2010.igem.org/Team:Panama/7_October_2010 7]	[http://2010.igem.org/Team:Panama/8_October_2010 8]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/9_October_2010&action=edit 9]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/10_October_2010&action=edit 10]
[http://2010.igem.org/Team:Panama/11_October_2010 11]	[http://2010.igem.org/Team:Panama/12_October_2010 12]	[http://2010.igem.org/Team:Panama/13_October_2010 13]	[http://2010.igem.org/Team:Panama/14_October_2010 14]	[http://2010.igem.org/Team:Panama/15_October_2010 15]	[http://2010.igem.org/Team:Panama/16_October_2010 16]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/17_October_2010&action=edit 17]
[http://2010.igem.org/Team:Panama/18_October_2010 18]	[http://2010.igem.org/Team:Panama/19_October_2010 19]	[http://2010.igem.org/Team:Panama/20_October_2010 20]	[http://2010.igem.org/Team:Panama/21_October_2010 21]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/22_October_2010&action=edit 22]	[http://2010.igem.org/Team:Panama/23_October_2010 23]	[http://2010.igem.org/Team:Panama/24_October_2010 24]
[http://2010.igem.org/Team:Panama/25_October_2010 25]	[http://2010.igem.org/Team:Panama/26_October_2010 26]	[http://2010.igem.org/Team:Panama/27_October_2010 27]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/28_October_2010&action=edit 28]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/29_October_2010&action=edit 29]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/30_October_2010&action=edit 30]	[http://2010.igem.org/wiki/index.php?title=Team:Panama/31_October_2010&action=edit 31]