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Team:Panama/4 October 2010
From 2010.igem.org
(Difference between revisions)
(New page: Ligation of Reporter and Terminator (About two hours) We used the same quantities that were used on September 23 2010 PCR (About 2.5 hours) On October 1, we did the miniprep of the clo...) |
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Today, we will do a PCR of this to see whether the clones that have grown contain the gene Rh1ab | Today, we will do a PCR of this to see whether the clones that have grown contain the gene Rh1ab | ||
| + | |||
| + | *Photo | ||
| + | |||
| + | No bands appear | ||
| + | |||
| + | Possible reasons: | ||
| + | |||
| + | 1) The PCR has had some sort of problem | ||
| + | |||
| + | 2) The cloning has had some sort of problem | ||
| + | |||
| + | Possible solutions: | ||
| + | |||
| + | 1) Repeat the PCR, changing the denaturizing temperature time to 8 minutes and in the cycling from 30 seconds to 1 minute. Besides that, we used two different miniprep samples from the cloning (maybe the colony that we picked doesn't have the insert) | ||
| + | |||
| + | *Photo | ||
Revision as of 22:35, 21 October 2010
Ligation of Reporter and Terminator (About two hours)
We used the same quantities that were used on September 23 2010
PCR (About 2.5 hours)
On October 1, we did the miniprep of the cloning product of the gene Rh1ab in the pGEM-T vector
Today, we will do a PCR of this to see whether the clones that have grown contain the gene Rh1ab
- Photo
No bands appear
Possible reasons:
1) The PCR has had some sort of problem
2) The cloning has had some sort of problem
Possible solutions:
1) Repeat the PCR, changing the denaturizing temperature time to 8 minutes and in the cycling from 30 seconds to 1 minute. Besides that, we used two different miniprep samples from the cloning (maybe the colony that we picked doesn't have the insert)
- Photo
