Team:Osaka/Tests

From 2010.igem.org

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<h2>Tests</h2>
<h2>Tests</h2>
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<ul>
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<h3>Cellulase assay: CMC clearing</h3>
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<li>promoters
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<h3>Microscope observation of cell surface display</h3>
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<li>terminators
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<h3>Cellulase quantitative activity assay: DNS method</h3>
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<li>yeast plasmid elements
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<li>secretion
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<h3>Future work</h3>
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<li>surface display
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<p>We created many parts (yeast ENO2 promoter, ADH1 terminator, SUC2 secretion signal etc as well as various cellulases such as xylanase and mannase) but did not have time to evaluate them. Also, devices containing 2 or more cellulases should have been constructed and assayed.</p>  
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<li>cellulose degradation
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<li>poly(gamma-glutamate)
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</ul>
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</div>
</div>
<div class="clear"><hr></div>
<div class="clear"><hr></div>

Revision as of 18:37, 27 October 2010


Tests

Cellulase assay: CMC clearing

Microscope observation of cell surface display

Cellulase quantitative activity assay: DNS method

Future work

We created many parts (yeast ENO2 promoter, ADH1 terminator, SUC2 secretion signal etc as well as various cellulases such as xylanase and mannase) but did not have time to evaluate them. Also, devices containing 2 or more cellulases should have been constructed and assayed.


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