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Team:Northwestern/References
From 2010.igem.org
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=== Lac Operon Control === | === Lac Operon Control === | ||
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chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311 | chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311 | ||
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| + | ====Chitin Staining (Calcofluor)==== | ||
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| + | Does not stain E Coli - http://www.sigmaaldrich.com/etc/medialib/docs/Fluka/Datasheet/18909dat.pdf | ||
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| + | Stains Biofilm Polysaccharides? - http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6V73-3YKKH2J-4S-1&_cdi=5831&_user=965532&_pii=0043135494003399&_orig=search&_coverDate=08%2F31%2F1995&_sk=999709991&view=c&wchp=dGLbVzz-zSkzS&md5=fedcb287ed9c9dafca2dbb4f2bc053a4&ie=/sdarticle.pdf | ||
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| + | Inhibits Synthase - http://www.ncbi.nlm.nih.gov/pmc/articles/instance/40653/ | ||
=== Apoptosis === | === Apoptosis === | ||
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registry: http://partsregistry.org/Cell_death | registry: http://partsregistry.org/Cell_death | ||
| - | + | ==== Live/Dead Staining aka Viability/Cytotoxicity Assay | |
| + | DMAO(Live), EtD-III(Dead) - http://www.promokine.info/products/cell-analysis/cell-staining-reagents-related-products/, http://www.biotium.com/product/product_info/Protocol/30027.pdf | ||
| - | === Chitinase Knockout (may not be necessary) === | + | |
| + | ==Keio Collection== | ||
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| + | http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp | ||
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| + | === ChiA(Chitinase) Knockout (may not be necessary) === | ||
summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts | summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts | ||
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chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300 | chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300 | ||
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| + | ===ydgg/tqsA (AI2-Transporter) Knockout=== | ||
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| + | KO -> ~7000x biofilm thickness, ~500x biomass - http://jb.asm.org/cgi/content/short/188/2/587 | ||
Revision as of 16:04, 28 June 2010
| Home | Brainstorm | Team | Acknowledgements | Project | Side Project | Parts | Notebook | Calendar | Protocol | Safety | Links | References | Media | Contact |
|---|
Contents |
Lac Operon Control
http://openwetware.org/wiki/IPTG
Chitin Synthesis
chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311
Chitin Staining (Calcofluor)
Does not stain E Coli - http://www.sigmaaldrich.com/etc/medialib/docs/Fluka/Datasheet/18909dat.pdf
Stains Biofilm Polysaccharides? - http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6V73-3YKKH2J-4S-1&_cdi=5831&_user=965532&_pii=0043135494003399&_orig=search&_coverDate=08%2F31%2F1995&_sk=999709991&view=c&wchp=dGLbVzz-zSkzS&md5=fedcb287ed9c9dafca2dbb4f2bc053a4&ie=/sdarticle.pdf
Inhibits Synthase - http://www.ncbi.nlm.nih.gov/pmc/articles/instance/40653/
Apoptosis
registry: http://partsregistry.org/Cell_death
==== Live/Dead Staining aka Viability/Cytotoxicity Assay
DMAO(Live), EtD-III(Dead) - http://www.promokine.info/products/cell-analysis/cell-staining-reagents-related-products/, http://www.biotium.com/product/product_info/Protocol/30027.pdf
Keio Collection
http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp
ChiA(Chitinase) Knockout (may not be necessary)
summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts
chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837
chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300
ydgg/tqsA (AI2-Transporter) Knockout
KO -> ~7000x biofilm thickness, ~500x biomass - http://jb.asm.org/cgi/content/short/188/2/587
