Team:Northwestern/Protocol

From 2010.igem.org

(Difference between revisions)
(Reagents)
(Reagents)
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=='''Reagents'''==
=='''Reagents'''==
-
==[[Reagents]]==
+
[[Reagents]]
=='''Cell Staining'''==
=='''Cell Staining'''==
===[[Rhodamine-Phalloidin/Calcofluor Staining]]===
===[[Rhodamine-Phalloidin/Calcofluor Staining]]===
===[[LIVE/DEAD® BacLight - Bacterial Viability Kit]]===
===[[LIVE/DEAD® BacLight - Bacterial Viability Kit]]===

Revision as of 16:22, 29 June 2010

Tech Institute

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Contents

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

Assembly

Restriction Enzyme Digests

3A Assembly

Ligations

Mini Prep

Microscopy

Confocal Microscopy

Fluorescence Microscopy

Reagents

Reagents

Cell Staining

Rhodamine-Phalloidin/Calcofluor Staining

LIVE/DEAD® BacLight - Bacterial Viability Kit