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Revision as of 04:22, 18 June 2010 (view source) Cyu286 (Talk | contribs) (→chitinase knockout) ← Older edit		Revision as of 04:22, 18 June 2010 (view source) Cyu286 (Talk | contribs) (→chitinase knockout) Newer edit →
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	http://www.ncbi.nlm.nih.gov/gene/947837		http://www.ncbi.nlm.nih.gov/gene/947837
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		+	http://ecoli.naist.jp/GB6/info.jsp?id=JW3300
	=== holin ===		=== holin ===

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Revision as of 04:22, 18 June 2010

Home	Team	Project	Parts	Notebook	Safety	Calendar	Protocol

Contents 1 Overall project 2 Project Details 2.1 volatile cell signalling 2.2 non-volatile cell signalling 2.3 chitin synthase 2.4 chitinase knockout 2.5 holin 3 Results

Overall project

Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)

in chitinase-KO strain e coli

Project Details

volatile cell signalling

phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA

http://www.ncbi.nlm.nih.gov/pubmed/9171411?dopt=Abstract

http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411

non-volatile cell signalling

http://partsregistry.org/Cell-cell_signalling quorum sensing: LuxR, LuxS, LuxpR

chitin synthase

http://www.ncbi.nlm.nih.gov/gene/852311

UDP-GlcNAc -> UDP + Chitin

chitinase knockout

http://openwetware.org/wiki/Escherichia_coli/Knockouts

http://www.ncbi.nlm.nih.gov/gene/947837

http://ecoli.naist.jp/GB6/info.jsp?id=JW3300

holin

http://partsregistry.org/Cell_death

Results