Team:Newcastle/solution

From 2010.igem.org

(Difference between revisions)
(Required BioBricks)
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{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
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Put explanation of alkaline resistant ''Bacillus subtilis'' growth on the wiki, maybe repeat the experiment.
 
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== Initial Research==  
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==Overview==
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{|
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...
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|[[Team:Newcastle/research|Research]]
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|[[Team:Newcastle/Initial_filamentous|Initial Research on Filamentous cells]]
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|}
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== Required BioBricks ==
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==BioBricks ==
#[[Team:Newcastle/Urease|CaCO<sub>3</sub>/Urease]]
#[[Team:Newcastle/Urease|CaCO<sub>3</sub>/Urease]]
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#[[Team:Newcastle/glue|Glue]]
#[[Team:Newcastle/glue|Glue]]
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==Parts Submitted to the Registry==
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===Parts Submitted to the Registry===
<html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Newcastle" height="700px" width="100%" ></iframe></html>
<html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Newcastle" height="700px" width="100%" ></iframe></html>
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==Alkalinity resistance==
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...Concrete has high pH ...explanation, the solution, link to the lab book experiments.
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== Initial Research==
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{|
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|[[Team:Newcastle/research|Research]]
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|-
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|[[Team:Newcastle/Initial_filamentous|Initial Research on Filamentous cells]]
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|}
== Characteristics / Actions ==
== Characteristics / Actions ==

Revision as of 16:00, 19 September 2010

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Contents

Overview

...

BioBricks

  1. CaCO3/Urease
  2. yneA
  3. End of crack & signalling system
  4. Swarming
  5. Non-target-environment kill switch
  6. Glue

Parts Submitted to the Registry

Alkalinity resistance

...Concrete has high pH ...explanation, the solution, link to the lab book experiments.

Initial Research

Research
Initial Research on Filamentous cells

Characteristics / Actions

  • Alkaline resistant
  • Motility
  • Sense Oxygen (for cracks)
  • Quorum Sensing
  • UV Sensitivity
  • Produce glue/Silk
  • Produce product in response to signal


Environment

  • Fermenter (Batch)
  • External Face of Concrete
  • Inside Crack
  • Spray
  • End of crack
  • Non-target

Experiments

  1. Movement into cracks
  2. Speed of swimming in a particular direction


Parameters

  1. Speed of swimming in a particular direction (wt)
  2. Quantities of glue, s.s, CaCO3, cell density
  3. Strength (tensile) of bonding agents, filamentous cells


 ???

  1. Grown in fermenter, rich medium
    • Rods
  2. Sporulation medium
    • spores
  3. Spray canister
    • spores
  4. Sprayed on concrete surface
    • germinate -> rods
  5. In crack
    • rods
  6. End of crack
    • Produce glue/CaCO3/silk/filaments
    • Produce signal peptides
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