Team:Newcastle/Transformation of B. subtilis

From 2010.igem.org

Revision as of 15:19, 5 August 2010 by Alankoh (Talk | contribs)

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Transformation of Bacillus subtilis

Materials required

  1. Bacillus subtilis 168
  2. 10 µl of DNA
  3. SMM medium (1 liter)
    • 2.0 g of ammonium sulphate
    • 14.0g of dipotassium hydrogen phosphate
    • 6.0g of potassium dihydrogen phosphate
    • 1.0g of sodium citrate dehydrate
    • 0.2g of magnesium sulphate
    • Top up the rest of the medium with water
  4. MM competence medium
    • 10 ml of SMM medium
    • 125 µl of Solution E (40% glucose)
    • 100 µl of Tryptophane solution (at a concentration of 2mg/ml) –
    • 60 µl of Solution F (1M MgSO4)
    • 10 µl of 20% Casamino acids
    • 5 µl of 0.22% Fe-NH4-citrate
  5. Starvation medium
    • 10 ml of SMM medium
    • 125 µl of Solution E (40% glucose)
    • 60 µl of Solution F (1M MgSO4)

Protocol

Go back to our Protocol List

Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon