Team:Newcastle/Transformation of B. subtilis

From 2010.igem.org

(Difference between revisions)
(Materials required)
Line 13: Line 13:
#* 0.2g of magnesium sulphate
#* 0.2g of magnesium sulphate
#* Top up the rest of the medium with water  
#* Top up the rest of the medium with water  
-
 
+
# MM competence medium
-
 
+
#* 10 ml of SMM medium
-
 
+
#* 125 µl of Solution E (40% glucose)
 +
#* 100 µl of Tryptophane solution (at a concentration of 2mg/ml) –
 +
#* 60 µl of Solution F (1M MgSO4)
 +
#* 10 µl of 20% Casamino acids
 +
#* 5 µl of 0.22% Fe-NH4-citrate

Revision as of 12:11, 5 August 2010

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Transformation of Bacillus subtilis

Materials required

  1. Bacillus subtilis 168
  2. 10 µl of DNA
  3. SMM medium (1 liter)
    • 2.0 g of ammonium sulphate
    • 14.0g of dipotassium hydrogen phosphate
    • 6.0g of potassium dihydrogen phosphate
    • 1.0g of sodium citrate dehydrate
    • 0.2g of magnesium sulphate
    • Top up the rest of the medium with water
  4. MM competence medium
    • 10 ml of SMM medium
    • 125 µl of Solution E (40% glucose)
    • 100 µl of Tryptophane solution (at a concentration of 2mg/ml) –
    • 60 µl of Solution F (1M MgSO4)
    • 10 µl of 20% Casamino acids
    • 5 µl of 0.22% Fe-NH4-citrate




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