Team:Newcastle/Transformation of B. subtilis

From 2010.igem.org

(Difference between revisions)
Line 2: Line 2:
=Transformation of ''Bacillus subtilis''=
=Transformation of ''Bacillus subtilis''=
 +
 +
==Materials required==
 +
# ''Bacillus subtilis'' 168
 +
# 10 µl of DNA
 +
# SMM medium (1 liter)
 +
#* 2.0 g of ammonium sulphate
 +
#* 14.0g of dipotassium hydrogen phosphate
 +
#* 6.0g of potassium dihydrogen phosphate
 +
#* 1.0g of sodium citrate dehydrate
 +
#* 0.2g of magnesium sulphate
 +
#* Top up the rest of the medium with water
 +
 +
 +
 +

Revision as of 12:06, 5 August 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Transformation of Bacillus subtilis

Materials required

  1. Bacillus subtilis 168
  2. 10 µl of DNA
  3. SMM medium (1 liter)
    • 2.0 g of ammonium sulphate
    • 14.0g of dipotassium hydrogen phosphate
    • 6.0g of potassium dihydrogen phosphate
    • 1.0g of sodium citrate dehydrate
    • 0.2g of magnesium sulphate
    • Top up the rest of the medium with water





Go back to our Protocol List

Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon