Team:Newcastle/Slide Preparation

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#Grow overnight culture in 5ml of LB broth at 37C
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#Dilute the overnight culture to a ratio of 1:100 and incubate at 37C for 1 hour
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#Set up the following broths with different concentrations of IPTG in 5ml of LB broth
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* 2mM IPTG
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* 0.2mM IPTG
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* 0.02mM IPTG
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* Broth only
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{{Team:Newcastle/footer}}

Revision as of 08:24, 23 September 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map
  1. Grow overnight culture in 5ml of LB broth at 37C
  2. Dilute the overnight culture to a ratio of 1:100 and incubate at 37C for 1 hour
  3. Set up the following broths with different concentrations of IPTG in 5ml of LB broth
  • 2mM IPTG
  • 0.2mM IPTG
  • 0.02mM IPTG
  • Broth only













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