Team:Newcastle/Scanning EM


Revision as of 22:02, 27 October 2010 by Swoodhouse (Talk | contribs)

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Scanning EM

This is a standard method for Scanning Electron Microscopy. Specimens vary greatly and the final protocol should be decided by referring to text books and publications.


Fix in 2% Gluteraldehyde in Sorensons Phosphate Buffer overnight (minimum).

Rinse in Sorensons Phosphate Buffer 2x 15 mins.


  • 25% ethanol: 30 mins.
  • 50% ethanol: 30 mins.
  • 75% ethanol: 30 mins.
  • 100% ethanol: 1 hr.
  • 100% ethanol: 1 hr.

Once in 100% ethanol, samples are dried using a Baltec Critical Point Dryer.


Mount of an aluminium stub with Achesons Silver ElectroDag.

Gold coating

Coat with 15nm of gold using a Polaron SEM Coating Unit.

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