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Team:Newcastle/Restriction digests
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To cut DNA at specific base sequences using restriction enzymes | To cut DNA at specific base sequences using restriction enzymes | ||
Revision as of 09:08, 27 July 2010
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To cut DNA at specific base sequences using restriction enzymes
Materials
The following are required to make a 30 µl solution:
- 1 µl of restriction enzymes in total
- 3 µl of 10x buffer
- remainder is DNA/water
Notes
- No more than 10% of enzyme - solution contains glycerol which inhibits reaction
- 10x buffer must be diluted to 1x i.e. 10% final volume
Methods
- Load desired quantities of solution into microfuge tube
- Incubate at 37°C for 3 hours.
