Team:Newcastle/Protocol list

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Our Protocol List

This page contains the many protocols used by Team Newcastle 2010!

• PCR
• Gibson cloning
• Preparing competent E. coli
• Transformation of E. coli
• Nanodrop Spectrophotometer
• Growing an overnight culture
• Minipreps
• Ligation
• Restriction digests
• Gel electrophoresis
• Transformation of Bacillus subtilis
• DNA extraction of Bacillus subtilis
• Arginine test
• Urease test
• Preparing competent Bacillus subtilis