Team:Newcastle/Nanodrop

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(New page: ==Nanodrop== ===Materials required=== # Water # Buffer used to resuspend the sample # Nanodrop machine # Pipettes ===Procedures=== # Select Nanodrop program from the desktop # To clean ...)
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===Materials required===
===Materials required===
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# Water
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* Water
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# Buffer used to resuspend the sample
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* Buffer used to resuspend the sample
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# Nanodrop machine
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* Nanodrop machine
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# Pipettes
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* Pipettes
===Procedures===
===Procedures===

Revision as of 14:44, 28 July 2010

Nanodrop

Materials required

  • Water
  • Buffer used to resuspend the sample
  • Nanodrop machine
  • Pipettes

Procedures

  1. Select Nanodrop program from the desktop
  2. To clean Nanodrop, add a drop of water on the spectrometer and press blank
  3. After cleaning, wipe the water off
  4. To equalizen the equipment, add 3 μl of the buffer used in the sample and press blank
  5. Wipe the buffer off
  6. To measure sample, add 3 μl of the sample and press measure
  7. If dealing with multiple samples, clean the equipment with water at regular intervals
  8. After measurement, clean the equipment with a drop of water on the spectrometer and press blank