Team:Newcastle/Minipreps
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RachelBoyd (Talk | contribs) (New page: ==Minipreps== ===Gel extraction=== #Excise the DNA fragment from the agarose gel with a clean, sharp scalpel #Weigh the gel slice in the colourless tube. Add 3 volumes Buffer QG to 1 vol...) |
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Revision as of 13:37, 28 July 2010
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Minipreps
Gel extraction
- Excise the DNA fragment from the agarose gel with a clean, sharp scalpel
- Weigh the gel slice in the colourless tube. Add 3 volumes Buffer QG to 1 volume of gel slice in a colourless tube (100mg~10µl)
- Incubate at 50°C for 10 min (or until the gel slice has completely dissolved)vortex every 2-3minutes
- After the gel slice has dissolved completely, check the colour of the mixture is yellow
- Add 1gell volume of isopropanol to the sample and mix
- Place QG quick spin column in 2ml collection tube
- Add sample and spin for 1 minute (discard flow through)
- Add QG buffer and spin for 1min (discard flow through)
- Add PE buffer and spin for 1min (discard flow through)(13000rpm)
- Place spin column in clean tube(1.5ml)
- Add elution buffer (50µl)spin for 1 min
- If using a gel to analyse add loading dye and mix (pipet up and down)