Team:Newcastle/Meetings/4 June 2010

From 2010.igem.org

(Difference between revisions)
(Informal Meeting - 04.06.2010)
 
(3 intermediate revisions not shown)
Line 5: Line 5:
* [[Team:Newcastle/Agendas/4_June_2010|Agenda]]
* [[Team:Newcastle/Agendas/4_June_2010|Agenda]]
-
====Urease tutorial-recap====
+
====Urease tutorial - recap====
* Accessory genes and nickel incorporation necessary more complicated than we thought.  
* Accessory genes and nickel incorporation necessary more complicated than we thought.  
* Software engineering cycle  
* Software engineering cycle  
Line 29: Line 29:
* [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_10:_Draft_standard_for_Biobrick_biological_parts duplo and lego don’t fit together]
* [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_10:_Draft_standard_for_Biobrick_biological_parts duplo and lego don’t fit together]
* [http://www.youtube.com/watch?v=FRijFX0BpGI&feature=player_embedded Video for lego pipette]
* [http://www.youtube.com/watch?v=FRijFX0BpGI&feature=player_embedded Video for lego pipette]
-
 
-
====1st lab week==== (Others modelling Filamentous and spidersilk killswitch)           
 
-
* RC
 
-
* DY
 
-
* AK
 
-
* (HS)
 
-
 
-
====2nd lab week==== (Others Modelling glue and lysis quorum sensing)
 
-
*JS
 
-
*RB
 
-
*SW
 
-
*PH
 
-
*(YE)
 
-
*Urease both
 
====LAB TRAINING GROUPS:====
====LAB TRAINING GROUPS:====
Line 51: Line 37:
* All team members working on BioBrick 1 (CaCO3/Urease).
* All team members working on BioBrick 1 (CaCO3/Urease).
* RC working on BioBrick 6 (sequential programming)
* RC working on BioBrick 6 (sequential programming)
-
 
* Team members not in the lab training will be working on modeling.
* Team members not in the lab training will be working on modeling.

Latest revision as of 09:01, 27 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Informal Meeting - 4 June 2010

Urease tutorial - recap

  • Accessory genes and nickel incorporation necessary more complicated than we thought.
  • Software engineering cycle
  • Requirements -> specification-> design-> implementation -> testing -> maintenance ...link back to the requirements
  • Specification more detail on the requirements- literature
  • Urease calcium carbonate, optimise for Bacillis
  • Increase expression- what we thought!
  • Biosynthesis of active BS in the Absence of Known Urease Accessory Proteins: paper
  • Accessory genes required!
  • Look over papers from tutorial
  • Sporosarcina pasteurii
  • Biobrick site symbols
  • Promoter-ribosome- binding- coding sequence –ribosome binding site-coding-ribosome binding coding- terminator sequences ...and spacers
  • pSpac
  • ncbi – find the genome – can see the genes
  • Implementation using sequencher
  • Find the sequence of part
  • Assemble parts
  • Biobrick friendly restriction sites
  • Genbank/embl file format
  • Implementation altered by cloning strategy
  • Biobricks RFC- request for comments
  • duplo and lego don’t fit together
  • Video for lego pipette

LAB TRAINING GROUPS:

  • Group 1 starts 14th June: SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis)
  • Group 2 starts 21st June: JS, RB, RC, PH, (YE); working on BioBrick 3 & 4 (Non-target-environment kill switch; Spider silk and filamentous cells)
  • All team members working on BioBrick 1 (CaCO3/Urease).
  • RC working on BioBrick 6 (sequential programming)
  • Team members not in the lab training will be working on modeling.
  • HS and YE will unfortunately be away for both lab training sessions, hence the brackets
Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon