Team:Newcastle/Meetings/26 March 2010

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==Formal meeting - 26th March 2010==  
==Formal meeting - 26th March 2010==  
* Room: CBCB; Start: 11 am
* Room: CBCB; Start: 11 am
Line 8: Line 6:
*Apologies: Phil (Holiday), Jem and Matt  
*Apologies: Phil (Holiday), Jem and Matt  
*Approval of agenda and minutes - Approved  
*Approval of agenda and minutes - Approved  
-
3. Brief summary of previous meeting
 
-
|-
 
-
|Tutorial
 
-
|-
 
-
  | 4. Research Feedback
 
-
|-
 
-
        |1. Properties of concrete and surface tension (Zoltan and Steven)
 
-
High compressive strength low tensile strength
 
-
Steal added good for temp change
 
-
Caco3 – similar to concrete; expand same as concrete... might crack like concrete
 
-
Spider silk like steal- reduce possibility of cracking 
 
-
Glue- no many papers- expansion may not be a problem? 
 
-
Silk alkali resistant
 
-
Capillary properties of concrete change over time- in project we consider small rapid
 
-
Cement water ratio and surface area and environment
 
-
Where to look for the cracks? Crack pattern
 
-
|-
 
-
        | 2. Microinjection (Zoltan and Steven)
 
-
|-
 
-
|Simple issue
 
-
Not correct term
 
-
Swimming Bacillus
 
-
Oxygen gradient big selling points
 
-
|-
 
-
        |3. Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)
 
-
|-
 
-
|Test in plasmids- Find good plasmid
 
-
Integrate into genome 
 
-
Recombination site... Find!!!
 
-
amyE site amylase with insert can’t break down amylase
 
-
|-
 
-
        |4. Nutrients (Harsh and Rachel)
 
-
|-
 
-
|Bacteria algae growth living in concrete
 
-
Only activate feed bacillus
 
-
Two hybrid
 
-
Bio films exclude other not algae
 
-
Antifungals, anti algals, Antibiotic
 
-
|-
 
-
        |5. Sporulation, germination and secretion control (Alan and Da)
 
-
|-
 
-
|SpoA – last years team sporulation control, maybe turn off completely
 
-
Nutrient availability
 
-
KinA
 
-
Inducible promoter
 
-
|-
 
-
|Glue out of bug?
 
-
|-
 
-
        |6. Quorum sensing (Younus and Phil)
+
====4. Research Feedback====
-
|-
+
'''Properties of concrete and surface tension''' (Zoltan and Steven)
-
|Compx
+
*High compressive strength low tensile strength
-
And cff
+
*Steal added good for temp change
-
Two component system comp
+
*CaCO<sub>3</sub> – similar to concrete; expand same as concrete... might crack like concrete
-
|-
+
*Spider silk like steal- reduce possibility of cracking 
-
        |-7. Kill switch (Richard and Jannetta)
+
*Glue- no many papers- expansion may not be a problem? 
-
|-
+
*Silk alkali resistant
-
|Why ? Don’t want?- not die after job fill new cracks  
+
*Capillary properties of concrete change over time- in project we consider small rapid
-
Nuclease  
+
*Cement water ratio and surface area and environment
-
Phil masEF  
+
*Where to look for the cracks? Crack pattern
-
Skf SbB  
+
'''Microinjection''' (Zoltan and Steven)
-
If linear DNA works  
+
*Simple issue
 +
*Not correct term
 +
*Swimming Bacillus
 +
*Oxygen gradient big selling points
 +
'''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel)
 +
*Test in plasmids- Find good plasmid
 +
*Integrate into genome 
 +
*Recombination site... Find!!!
 +
*amyE site amylase with insert can’t break down amylase
 +
'''Nutrients''' (Harsh and Rachel)
 +
*Bacteria algae growth living in concrete
 +
*Only activate feed bacillus
 +
*Two hybrid
 +
*Biofilms exclude other not algae
 +
*Antifungals, antialgaes, antibiotic
 +
'''Sporulation, germination and secretion control''' (Alan and Da)
 +
*SpoA – last years team sporulation control, maybe turn off completely
 +
*Nutrient availability
 +
*KinA
 +
*Inducible promoter
 +
*Glue out of bug
 +
'''Quorum sensing''' (Younus and Phil)
 +
*CompX and cff
 +
*Two-component system comP
 +
'''Kill switch''' (Richard and Jannetta)
 +
*Why ? Don’t want?- not die after job fill new cracks  
 +
*Nuclease  
 +
*Phil masEF  
 +
*Skf SbB  
 +
*If linear DNA works  
|-
|-

Revision as of 00:28, 26 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Formal meeting - 26th March 2010

  • Room: CBCB; Start: 11 am
  • Chair: Jannetta, Minutes: Rachel, Computer: Harsh
  • Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
  • Apologies: Phil (Holiday), Jem and Matt
  • Approval of agenda and minutes - Approved

4. Research Feedback

Properties of concrete and surface tension (Zoltan and Steven)

  • High compressive strength low tensile strength
  • Steal added good for temp change
  • CaCO3 – similar to concrete; expand same as concrete... might crack like concrete
  • Spider silk like steal- reduce possibility of cracking
  • Glue- no many papers- expansion may not be a problem?
  • Silk alkali resistant
  • Capillary properties of concrete change over time- in project we consider small rapid
  • Cement water ratio and surface area and environment
  • Where to look for the cracks? Crack pattern

Microinjection (Zoltan and Steven)

  • Simple issue
  • Not correct term
  • Swimming Bacillus
  • Oxygen gradient big selling points

Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)

  • Test in plasmids- Find good plasmid
  • Integrate into genome 
  • Recombination site... Find!!!
  • amyE site amylase with insert can’t break down amylase

Nutrients (Harsh and Rachel)

  • Bacteria algae growth living in concrete
  • Only activate feed bacillus
  • Two hybrid
  • Biofilms exclude other not algae
  • Antifungals, antialgaes, antibiotic

Sporulation, germination and secretion control (Alan and Da)

  • SpoA – last years team sporulation control, maybe turn off completely
  • Nutrient availability
  • KinA
  • Inducible promoter
  • Glue out of bug

Quorum sensing (Younus and Phil)

  • CompX and cff
  • Two-component system comP

Kill switch (Richard and Jannetta)

  • Why ? Don’t want?- not die after job fill new cracks
  • Nuclease
  • Phil masEF
  • Skf SbB
  • If linear DNA works

|-

        |8. Glue, CaCo3 and spider silk (Zoltan and Jannetta)

Bacteria that produce CaCo3 from urea How secrete spider silk long complex proteins – how can we make it happen in bacillus Secretion systems |-

        |9. Other points

|- |Linear DNA – Richard Justifications of Linear Build it up the use. Mutations- How stop general questions Not specifically a problem – keep thinking about it though |-

  |5. Action points

|- |All -Read Voygt paper |- |All-Next week working off the proper wiki!!! |- |Janetta contact details doc in drop box |- |Janetta and Richard – Flow chart!!! |- |Reminder to neil and jen not welcome trust funding by 2nd of April? |- |Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design |- |Janetta- send us links for graphic design software- please |- |Transfer the wiki |-

   |1. Individual iGEM applications

|- |Phil hasn’t applied |- |Richard not accepted |- |Janetta text Phil |- |Jen/Neil-Pay for team!! |-


        |2. Visas/Passport update

|- |Harsh- May June apply |- |Richard visa waiver |-

        |3. Wiki transfer

|-

              |1. Clarify contents of identified pages

|-

              |2. Id Ethics page 

|- |Research page |- |Safety page |- |entify other pages required |-

              |3. Ideas for layout / theme

|- |Zoltan Tidy it up-Urgent |- |Borrow last years / Look at anything thats nice |- |It evolves!!! We can change it!!! Now!!!!!!! |-


    |4. Travel plans

|-

              |1. Flights

|- |Need to sort out who is going |-

              |2. Accomodation

|- |Need to sort out who is going |-

        |5. Funding

|- |Harsh has made draft letter |- |Main chemical company – free stuff ?

|-

        |6. Advisors/additional team members - civil engineer/graphics designer?

|- |Still looking –Neil,Jen |-

        |7. Tutorials

|- |Sequentia and Clone manager- basics |- |Modelling part two |-

        |8. What next?

|- |Flow chart of what we’re going to do Big picture!!! |- |If its coloured life is easier |- |Cambridge brick-Colour |- |Web services for Biosynthetics, Flow chart |- |Deverner |-

        |9. Time line discussion 

|- |Need someone else on time line. |- |16th of June Lab induction |- |Deadlines soon |- |Change flight deadline – not 31st

|-

|6. Other business

|- |Pdf mark up tools – pdf exchange viewer, foxit, keeps mark up |- |Making dropbox use better |-

  |7. Items for next agenda

|- |Need wiki transferred by next meeting!!! |- |Review of flow chart if done |-

  |8. Next meeting- Thursday early as possible – email organise 
        1. Chair-Da 
        2. Minutes-Alan
        3. Computer-Younus
|-