Team:Newcastle/Meetings/26 March 2010

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==Formal meeting - 26th March 2010==
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| Minutes for formal meeting 26th March 2010  
+
* Room: CBCB; Start: 11 am
-
|-
+
* Chair: Jannetta, Minutes: Rachel, Computer: Harsh  
-
|* Room: CBCB; Start: 11 am
+
*Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
-
|-
+
*Apologies: Phil (holiday), Jem and Matt  
-
    |* Chair: Jannetta, Minutes: Rachel, Computer: Harsh  
+
*Approval of agenda and minutes - Approved  
-
|-
+
*[[Team:Newcastle/Agendas/26_March_2010| Agenda]]
-
  |1. Roll call and apologies
+
-
|-
+
-
|Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
+
-
|-
+
-
|Phil – Holiday
+
-
|-
+
-
|Apologies - Jem and Matt  
+
-
|-
+
-
  | 2. Approval of agenda and minutes
+
-
|-
+
-
|Approved  
+
-
|-
+
-
  |3. Brief summary of previous meeting
+
-
|-
+
-
|Tutorial
+
-
|-
+
-
  | 4. Research Feedback
+
-
|-
+
-
        |1. Properties of concrete and surface tension (Zoltan and Steven)
+
-
High compressive strength low tensile strength
+
-
Steal added good for temp change
+
-
Caco3 – similar to concrete; expand same as concrete... might crack like concrete
+
-
Spider silk like steal- reduce possibility of cracking 
+
-
Glue- no many papers- expansion may not be a problem? 
+
-
Silk alkali resistant
+
-
Capillary properties of concrete change over time- in project we consider small rapid
+
-
Cement water ratio and surface area and environment
+
-
Where to look for the cracks? Crack pattern
+
-
|-
+
-
        | 2. Microinjection (Zoltan and Steven)
+
-
|-
+
-
|Simple issue
+
-
Not correct term
+
-
Swimming Bacillus
+
-
Oxygen gradient big selling points
+
-
|-
+
-
        |3. Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)
+
-
|-
+
-
|Test in plasmids- Find good plasmid
+
-
Integrate into genome 
+
-
Recombination site... Find!!!
+
-
amyE site amylase with insert can’t break down amylase
+
-
|-
+
-
        |4. Nutrients (Harsh and Rachel)
+
-
|-
+
-
|Bacteria algae growth living in concrete
+
-
Only activate feed bacillus
+
-
Two hybrid
+
-
Bio films exclude other not algae
+
-
Antifungals, anti algals, Antibiotic
+
-
|-
+
-
        |5. Sporulation, germination and secretion control (Alan and Da)
+
-
|-
+
-
|SpoA – last years team sporulation control, maybe turn off completely
+
-
Nutrient availability
+
-
KinA
+
-
Inducible promoter
+
-
|-
+
-
|Glue out of bug?
+
-
|-
+
-
        |6. Quorum sensing (Younus and Phil)
+
====Research Feedback====
-
|-
+
-
|Compx
+
-
And cff
+
-
Two component system comp
+
-
|-
+
-
        |-7. Kill switch (Richard and Jannetta)
+
-
|-
+
-
|Why ? Don’t want?- not die after job fill new cracks
+
-
Nuclease
+
-
Phil masEF
+
-
Skf SbB
+
-
If linear DNA works
+
-
|-
+
-
        |8. Glue, CaCo3 and spider silk (Zoltan and Jannetta)
+
#'''Properties of concrete and surface tension''' (Zoltan and Steven)
-
Bacteria that produce CaCo3 from urea   
+
#*High compressive strength low tensile strength
-
How secrete spider silk long complex proteins – how can we make it happen in bacillus
+
#*Steal added good for temp change
-
Secretion systems  
+
#*CaCO<sub>3</sub> – similar to concrete; expand same as concrete - might crack like concrete
-
|-
+
#*Spider silk like steal - reduce possibility of cracking 
-
        |9. Other points
+
#*Glue - no many papers - expansion may not be a problem? 
-
|-
+
#*Silk alkali resistant
-
|Linear DNA – Richard  
+
#*Capillary properties of concrete change over time- in project we consider small rapid
-
Justifications of Linear   
+
#*Cement water ratio and surface area and environment
-
Build it up the use.
+
#*Where to look for the cracks? Crack pattern
-
Mutations- How stop general questions
+
#'''Microinjection''' (Zoltan and Steven)
-
Not specifically a problem – keep thinking about it though  
+
#*Simple issue
-
|-
+
#*Not correct term
 +
#*Swimming ''Bacillus''
 +
#*Oxygen gradient big selling points
 +
#'''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel)
 +
#*Test in plasmids- Find good plasmid
 +
#*Integrate into genome
 +
#*Recombination site... Find!!!
 +
#*amyE site amylase with insert can’t break down amylase
 +
#'''Nutrients''' (Harsh and Rachel)
 +
#*Bacteria algae growth living in concrete
 +
#*Only activate feed bacillus
 +
#*Two hybrid
 +
#*Biofilms exclude other not algae
 +
#*Antifungals, antialgaes, antibiotic
 +
#'''Sporulation, germination and secretion control''' (Alan and Da)
 +
#*SpoA – last years team sporulation control, maybe turn off completely
 +
#*Nutrient availability
 +
#*KinA
 +
#*Inducible promoter
 +
#*Glue out of bug
 +
#'''Quorum sensing''' (Younus and Phil)
 +
#*CompX and cff
 +
#*Two-component system comP
 +
#'''Kill switch''' (Richard and Jannetta)
 +
#*Why ? Don’t want?- not die after job fill new cracks
 +
#*Nuclease
 +
#*Phil mazEF
 +
#*Skf SbB
 +
#*If linear DNA works
 +
#'''Glue, CaCo3 and spider silk''' (Zoltan and Jannetta)
 +
#*Bacteria that produce CaCo3 from urea   
 +
#*How secrete spider silk long complex proteins – how can we make it happen in bacillus
 +
#*Secretion systems  
 +
#'''Other points'''
 +
#*Linear DNA – Richard  
 +
#*Justifications of Linear   
 +
#*Build it up the use  
 +
#*Mutations
 +
#*Not specifically a problem – keep thinking about it though
-
  |5. Action points
+
====Action points====
-
|-
+
*All - Read Voigt paper  
-
|All -Read Voygt paper  
+
*All - Next week working off the proper wiki
-
|-
+
*Janetta contact details doc in drop box  
-
|All-Next week working off the proper wiki!!!
+
*Janetta and Richard – Flow chart!!!  
-
|-
+
*Reminder to neil and jen not welcome trust funding by 2nd of April?  
-
|Janetta contact details doc in drop box  
+
*Janetta - T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper, quotes dependent on design  
-
|-
+
*Jannetta - send us links for graphic design software- please
-
|Janetta and Richard – Flow chart!!!  
+
*Transfer the wiki  
-
|-
+
*Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
-
|Reminder to neil and jen not welcome trust funding by 2nd of April?  
+
*Jen/Neil - Pay for team!!
-
|-
+
*Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver
-
|Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design  
+
-
|-
+
-
|Janetta- send us links for graphic design software- please
+
-
|-
+
-
|Transfer the wiki  
+
-
|-
+
-
    |1. Individual iGEM applications
+
-
|-
+
-
|Phil hasn’t applied  
+
-
|-
+
-
|Richard not accepted  
+
-
|-
+
-
|Janetta text Phil  
+
-
|-
+
-
|Jen/Neil-Pay for team!!
+
-
|-
+
-
+
-
        |2. Visas/Passport update
+
====Wiki transfer====
-
|-
+
*Clarify contents of identified pages
-
|Harsh- May June apply
+
*Ethics page
-
|-
+
*Research page
-
|Richard visa waiver
+
*Safety page
-
|-
+
*Identify other pages required
-
        |3. Wiki transfer
+
*Ideas for layout/theme
-
|-
+
*Zoltan - Tidy it up - Urgent
-
              |1. Clarify contents of identified pages
+
*Borrow last years / Look at anything thats nice
 +
*It evolves!!! We can change it
 +
====Travel plans====
 +
*Flights and Accomodation
 +
**Need to sort out  who is going
 +
====Funding====
 +
*Harsh has made draft letter
 +
*Main chemical company – free stuff?
 +
====Advisors/additional team members - civil engineer/graphics designer====
 +
*Still looking – Neil, Jen
-
|-
+
====Tutorials====
-
              |2. Id Ethics page
+
#Sequentia and Clone manager - basics
-
|-
+
#Modelling part two
-
|Research page
+
====What next?====
-
|-
+
*Flow chart of what we’re going to do Big picture!!!
-
|Safety page
+
*If its coloured life is easier
-
|-
+
*Cambridge brick - Colour
-
|entify other pages required
+
*Web services for Biosynthetics, Flow chart
-
|-
+
*Taverner
 +
====Timeline discussion====
 +
*Need someone else on time line.
 +
*16th of June Lab induction
 +
*Deadlines soon
 +
*Change flight deadline – not 31st
-
              |3. Ideas for layout / theme
+
====Other business====
-
|-  
+
*PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up  
-
|Zoltan Tidy it up-Urgent
+
*Making dropbox use better
-
|-
+
-
|Borrow last years / Look at anything thats nice
+
-
|-
+
-
|It evolves!!! We can change it!!! Now!!!!!!!
+
-
|-
+
 +
====Items for next agenda====
 +
* Need wiki transferred by next meeting
 +
*Review of flow chart if done
-
   
+
====Next meeting====
-
    |4. Travel plans
+
*Thursday early as possible – email to organise  
-
|-
+
*Chair: Da, Minutes: Alan, Computer: Younus
-
              |1. Flights
+
-
|-
+
-
|Need to sort out who is going
+
-
|-
+
-
              |2. Accomodation
+
-
|-
+
-
|Need to sort out  who is going
+
-
|-
+
-
        |5. Funding
+
-
|-
+
-
|Harsh has made draft letter
+
-
|-
+
-
|Main chemical company – free stuff ?
+
-
 
+
-
|-
+
-
        |6. Advisors/additional team members - civil engineer/graphics designer?
+
-
|-
+
-
|Still looking –Neil,Jen
+
-
|-
+
-
 
+
-
        |7. Tutorials
+
-
|-
+
-
|Sequentia and Clone manager- basics
+
-
|-
+
-
|Modelling part two
+
-
|-
+
-
        |8. What next?
+
-
|-
+
-
|Flow chart of what we’re going to do Big picture!!!
+
-
|-
+
-
|If its coloured life is easier
+
-
|-
+
-
|Cambridge brick-Colour
+
-
|-
+
-
|Web services for Biosynthetics, Flow chart
+
-
|-
+
-
|Deverner
+
-
|-
+
-
        |9. Time line discussion
+
-
|-
+
-
|Need someone else on time line.
+
-
|-
+
-
|16th of June Lab induction
+
-
|-
+
-
|Deadlines soon
+
-
|-
+
-
|Change flight deadline – not 31st
+
-
+
-
|-
+
-
|6. Other business
+
-
|-
+
-
|Pdf mark up tools – pdf exchange viewer, foxit, keeps mark up
+
-
|-
+
-
|Making dropbox use better
+
-
|-
+
-
 
+
-
  |7. Items for next agenda
+
-
|-
+
-
|Need wiki transferred by next meeting!!!
+
-
|-
+
-
|Review of flow chart if done
+
-
|-
+
-
  |8. Next meeting- Thursday early as possible – email organise  
+
-
        1. Chair-Da  
+
-
        2. Minutes-Alan
+
-
        3. Computer-Younus
+
-
|-
+

Latest revision as of 10:24, 27 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Formal meeting - 26th March 2010

  • Room: CBCB; Start: 11 am
  • Chair: Jannetta, Minutes: Rachel, Computer: Harsh
  • Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
  • Apologies: Phil (holiday), Jem and Matt
  • Approval of agenda and minutes - Approved
  • Agenda

Research Feedback

  1. Properties of concrete and surface tension (Zoltan and Steven)
    • High compressive strength low tensile strength
    • Steal added good for temp change
    • CaCO3 – similar to concrete; expand same as concrete - might crack like concrete
    • Spider silk like steal - reduce possibility of cracking
    • Glue - no many papers - expansion may not be a problem?
    • Silk alkali resistant
    • Capillary properties of concrete change over time- in project we consider small rapid
    • Cement water ratio and surface area and environment
    • Where to look for the cracks? Crack pattern
  2. Microinjection (Zoltan and Steven)
    • Simple issue
    • Not correct term
    • Swimming Bacillus
    • Oxygen gradient big selling points
  3. Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)
    • Test in plasmids- Find good plasmid
    • Integrate into genome
    • Recombination site... Find!!!
    • amyE site amylase with insert can’t break down amylase
  4. Nutrients (Harsh and Rachel)
    • Bacteria algae growth living in concrete
    • Only activate feed bacillus
    • Two hybrid
    • Biofilms exclude other not algae
    • Antifungals, antialgaes, antibiotic
  5. Sporulation, germination and secretion control (Alan and Da)
    • SpoA – last years team sporulation control, maybe turn off completely
    • Nutrient availability
    • KinA
    • Inducible promoter
    • Glue out of bug
  6. Quorum sensing (Younus and Phil)
    • CompX and cff
    • Two-component system comP
  7. Kill switch (Richard and Jannetta)
    • Why ? Don’t want?- not die after job fill new cracks
    • Nuclease
    • Phil mazEF
    • Skf SbB
    • If linear DNA works
  8. Glue, CaCo3 and spider silk (Zoltan and Jannetta)
    • Bacteria that produce CaCo3 from urea
    • How secrete spider silk long complex proteins – how can we make it happen in bacillus
    • Secretion systems
  9. Other points
    • Linear DNA – Richard
    • Justifications of Linear
    • Build it up the use
    • Mutations
    • Not specifically a problem – keep thinking about it though

Action points

  • All - Read Voigt paper
  • All - Next week working off the proper wiki
  • Janetta contact details doc in drop box
  • Janetta and Richard – Flow chart!!!
  • Reminder to neil and jen not welcome trust funding by 2nd of April?
  • Janetta - T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper, quotes dependent on design
  • Jannetta - send us links for graphic design software- please
  • Transfer the wiki
  • Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
  • Jen/Neil - Pay for team!!
  • Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver

Wiki transfer

  • Clarify contents of identified pages
  • Ethics page
  • Research page
  • Safety page
  • Identify other pages required
  • Ideas for layout/theme
  • Zoltan - Tidy it up - Urgent
  • Borrow last years / Look at anything thats nice
  • It evolves!!! We can change it

Travel plans

  • Flights and Accomodation
    • Need to sort out who is going

Funding

  • Harsh has made draft letter
  • Main chemical company – free stuff?

Advisors/additional team members - civil engineer/graphics designer

  • Still looking – Neil, Jen

Tutorials

  1. Sequentia and Clone manager - basics
  2. Modelling part two

What next?

  • Flow chart of what we’re going to do Big picture!!!
  • If its coloured life is easier
  • Cambridge brick - Colour
  • Web services for Biosynthetics, Flow chart
  • Taverner

Timeline discussion

  • Need someone else on time line.
  • 16th of June Lab induction
  • Deadlines soon
  • Change flight deadline – not 31st

Other business

  • PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up
  • Making dropbox use better

Items for next agenda

  • Need wiki transferred by next meeting
  • Review of flow chart if done

Next meeting

  • Thursday early as possible – email to organise
  • Chair: Da, Minutes: Alan, Computer: Younus
Retrieved from "http://2010.igem.org/Team:Newcastle/Meetings/26_March_2010"