Team:Newcastle/Meetings/26 March 2010

From 2010.igem.org

(Difference between revisions)
(Formal meeting - 26th March 2010)
 
(4 intermediate revisions not shown)
Line 6: Line 6:
*Apologies: Phil (holiday), Jem and Matt  
*Apologies: Phil (holiday), Jem and Matt  
*Approval of agenda and minutes - Approved  
*Approval of agenda and minutes - Approved  
 +
*[[Team:Newcastle/Agendas/26_March_2010| Agenda]]
====Research Feedback====
====Research Feedback====
Line 47: Line 48:
#*Why ? Don’t want?- not die after job fill new cracks  
#*Why ? Don’t want?- not die after job fill new cracks  
#*Nuclease  
#*Nuclease  
-
#*Phil masEF
+
#*Phil mazEF
#*Skf SbB  
#*Skf SbB  
#*If linear DNA works  
#*If linear DNA works  
Line 57: Line 58:
#*Linear DNA – Richard  
#*Linear DNA – Richard  
#*Justifications of Linear   
#*Justifications of Linear   
-
#*Build it up the use.
+
#*Build it up the use  
-
#*Mutations- How stop general questions
+
#*Mutations
#*Not specifically a problem – keep thinking about it though
#*Not specifically a problem – keep thinking about it though
====Action points====
====Action points====
-
*All - Read Voygt paper  
+
*All - Read Voigt paper  
*All - Next week working off the proper wiki
*All - Next week working off the proper wiki
*Janetta contact details doc in drop box  
*Janetta contact details doc in drop box  
Line 72: Line 73:
*Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
*Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
*Jen/Neil - Pay for team!!
*Jen/Neil - Pay for team!!
-
*Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver  
+
*Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver
 +
 
====Wiki transfer====
====Wiki transfer====
*Clarify contents of identified pages
*Clarify contents of identified pages
Line 90: Line 92:
*Main chemical company – free stuff?
*Main chemical company – free stuff?
====Advisors/additional team members - civil engineer/graphics designer====
====Advisors/additional team members - civil engineer/graphics designer====
-
*Still looking – Neil,Jen  
+
*Still looking – Neil, Jen
 +
 
====Tutorials====
====Tutorials====
#Sequentia and Clone manager - basics  
#Sequentia and Clone manager - basics  
Line 100: Line 103:
*Web services for Biosynthetics, Flow chart
*Web services for Biosynthetics, Flow chart
*Taverner  
*Taverner  
-
====Time line discussion====
+
====Timeline discussion====
*Need someone else on time line.  
*Need someone else on time line.  
*16th of June Lab induction  
*16th of June Lab induction  
*Deadlines soon  
*Deadlines soon  
-
*Change flight deadline – not 31st  
+
*Change flight deadline – not 31st
-
+
 
====Other business====
====Other business====
*PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up  
*PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up  

Latest revision as of 10:24, 27 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Formal meeting - 26th March 2010

  • Room: CBCB; Start: 11 am
  • Chair: Jannetta, Minutes: Rachel, Computer: Harsh
  • Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
  • Apologies: Phil (holiday), Jem and Matt
  • Approval of agenda and minutes - Approved
  • Agenda

Research Feedback

  1. Properties of concrete and surface tension (Zoltan and Steven)
    • High compressive strength low tensile strength
    • Steal added good for temp change
    • CaCO3 – similar to concrete; expand same as concrete - might crack like concrete
    • Spider silk like steal - reduce possibility of cracking
    • Glue - no many papers - expansion may not be a problem?
    • Silk alkali resistant
    • Capillary properties of concrete change over time- in project we consider small rapid
    • Cement water ratio and surface area and environment
    • Where to look for the cracks? Crack pattern
  2. Microinjection (Zoltan and Steven)
    • Simple issue
    • Not correct term
    • Swimming Bacillus
    • Oxygen gradient big selling points
  3. Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)
    • Test in plasmids- Find good plasmid
    • Integrate into genome
    • Recombination site... Find!!!
    • amyE site amylase with insert can’t break down amylase
  4. Nutrients (Harsh and Rachel)
    • Bacteria algae growth living in concrete
    • Only activate feed bacillus
    • Two hybrid
    • Biofilms exclude other not algae
    • Antifungals, antialgaes, antibiotic
  5. Sporulation, germination and secretion control (Alan and Da)
    • SpoA – last years team sporulation control, maybe turn off completely
    • Nutrient availability
    • KinA
    • Inducible promoter
    • Glue out of bug
  6. Quorum sensing (Younus and Phil)
    • CompX and cff
    • Two-component system comP
  7. Kill switch (Richard and Jannetta)
    • Why ? Don’t want?- not die after job fill new cracks
    • Nuclease
    • Phil mazEF
    • Skf SbB
    • If linear DNA works
  8. Glue, CaCo3 and spider silk (Zoltan and Jannetta)
    • Bacteria that produce CaCo3 from urea
    • How secrete spider silk long complex proteins – how can we make it happen in bacillus
    • Secretion systems
  9. Other points
    • Linear DNA – Richard
    • Justifications of Linear
    • Build it up the use
    • Mutations
    • Not specifically a problem – keep thinking about it though

Action points

  • All - Read Voigt paper
  • All - Next week working off the proper wiki
  • Janetta contact details doc in drop box
  • Janetta and Richard – Flow chart!!!
  • Reminder to neil and jen not welcome trust funding by 2nd of April?
  • Janetta - T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper, quotes dependent on design
  • Jannetta - send us links for graphic design software- please
  • Transfer the wiki
  • Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
  • Jen/Neil - Pay for team!!
  • Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver

Wiki transfer

  • Clarify contents of identified pages
  • Ethics page
  • Research page
  • Safety page
  • Identify other pages required
  • Ideas for layout/theme
  • Zoltan - Tidy it up - Urgent
  • Borrow last years / Look at anything thats nice
  • It evolves!!! We can change it

Travel plans

  • Flights and Accomodation
    • Need to sort out who is going

Funding

  • Harsh has made draft letter
  • Main chemical company – free stuff?

Advisors/additional team members - civil engineer/graphics designer

  • Still looking – Neil, Jen

Tutorials

  1. Sequentia and Clone manager - basics
  2. Modelling part two

What next?

  • Flow chart of what we’re going to do Big picture!!!
  • If its coloured life is easier
  • Cambridge brick - Colour
  • Web services for Biosynthetics, Flow chart
  • Taverner

Timeline discussion

  • Need someone else on time line.
  • 16th of June Lab induction
  • Deadlines soon
  • Change flight deadline – not 31st

Other business

  • PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up
  • Making dropbox use better

Items for next agenda

  • Need wiki transferred by next meeting
  • Review of flow chart if done

Next meeting

  • Thursday early as possible – email to organise
  • Chair: Da, Minutes: Alan, Computer: Younus