Team:Newcastle/Meetings/26 March 2010

From 2010.igem.org

(Difference between revisions)
(4. Research Feedback)
(Formal meeting - 26th March 2010)
 
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Line 4: Line 4:
* Chair: Jannetta, Minutes: Rachel, Computer: Harsh  
* Chair: Jannetta, Minutes: Rachel, Computer: Harsh  
*Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
*Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
-
*Apologies: Phil (Holiday), Jem and Matt  
+
*Apologies: Phil (holiday), Jem and Matt  
*Approval of agenda and minutes - Approved  
*Approval of agenda and minutes - Approved  
 +
*[[Team:Newcastle/Agendas/26_March_2010| Agenda]]
-
====4. Research Feedback====
+
====Research Feedback====
#'''Properties of concrete and surface tension''' (Zoltan and Steven)
#'''Properties of concrete and surface tension''' (Zoltan and Steven)
#*High compressive strength low tensile strength
#*High compressive strength low tensile strength
#*Steal added good for temp change  
#*Steal added good for temp change  
-
#*CaCO<sub>3</sub> – similar to concrete; expand same as concrete... might crack like concrete  
+
#*CaCO<sub>3</sub> – similar to concrete; expand same as concrete - might crack like concrete  
-
#*Spider silk like steal- reduce possibility of cracking   
+
#*Spider silk like steal - reduce possibility of cracking   
-
#*Glue- no many papers- expansion may not be a problem?   
+
#*Glue - no many papers - expansion may not be a problem?   
#*Silk alkali resistant  
#*Silk alkali resistant  
#*Capillary properties of concrete change over time- in project we consider small rapid  
#*Capillary properties of concrete change over time- in project we consider small rapid  
#*Cement water ratio and surface area and environment  
#*Cement water ratio and surface area and environment  
#*Where to look for the cracks? Crack pattern  
#*Where to look for the cracks? Crack pattern  
-
 
#'''Microinjection''' (Zoltan and Steven)
#'''Microinjection''' (Zoltan and Steven)
#*Simple issue  
#*Simple issue  
#*Not correct term  
#*Not correct term  
-
#*Swimming Bacillus  
+
#*Swimming ''Bacillus''
#*Oxygen gradient big selling points  
#*Oxygen gradient big selling points  
-
 
#'''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel)
#'''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel)
#*Test in plasmids- Find good plasmid  
#*Test in plasmids- Find good plasmid  
-
#*Integrate into genome
+
#*Integrate into genome
#*Recombination site... Find!!!  
#*Recombination site... Find!!!  
#*amyE site amylase with insert can’t break down amylase  
#*amyE site amylase with insert can’t break down amylase  
-
 
#'''Nutrients''' (Harsh and Rachel)
#'''Nutrients''' (Harsh and Rachel)
#*Bacteria algae growth living in concrete  
#*Bacteria algae growth living in concrete  
Line 38: Line 36:
#*Biofilms exclude other not algae  
#*Biofilms exclude other not algae  
#*Antifungals, antialgaes, antibiotic  
#*Antifungals, antialgaes, antibiotic  
-
 
#'''Sporulation, germination and secretion control''' (Alan and Da)
#'''Sporulation, germination and secretion control''' (Alan and Da)
#*SpoA – last years team sporulation control, maybe turn off completely  
#*SpoA – last years team sporulation control, maybe turn off completely  
Line 45: Line 42:
#*Inducible promoter  
#*Inducible promoter  
#*Glue out of bug
#*Glue out of bug
-
 
+
#'''Quorum sensing''' (Younus and Phil)
-
'''Quorum sensing''' (Younus and Phil)
+
#*CompX and cff
-
*CompX and cff
+
#*Two-component system comP
-
*Two-component system comP
+
#'''Kill switch''' (Richard and Jannetta)
-
 
+
#*Why ? Don’t want?- not die after job fill new cracks  
-
'''Kill switch''' (Richard and Jannetta)
+
#*Nuclease  
-
*Why ? Don’t want?- not die after job fill new cracks  
+
#*Phil mazEF
-
*Nuclease  
+
#*Skf SbB  
-
*Phil masEF
+
#*If linear DNA works  
-
*Skf SbB  
+
#'''Glue, CaCo3 and spider silk''' (Zoltan and Jannetta)
-
*If linear DNA works  
+
#*Bacteria that produce CaCo3 from urea   
-
 
+
#*How secrete spider silk long complex proteins – how can we make it happen in bacillus
-
 
+
#*Secretion systems  
-
'''Glue, CaCo3 and spider silk''' (Zoltan and Jannetta)
+
#'''Other points'''
-
*Bacteria that produce CaCo3 from urea   
+
#*Linear DNA – Richard  
-
*How secrete spider silk long complex proteins – how can we make it happen in bacillus
+
#*Justifications of Linear   
-
*Secretion systems  
+
#*Build it up the use  
-
 
+
#*Mutations
-
'''Other points'''
+
#*Not specifically a problem – keep thinking about it though
-
*Linear DNA – Richard  
+
-
Justifications of Linear   
+
-
Build it up the use.
+
-
Mutations- How stop general questions
+
-
Not specifically a problem – keep thinking about it though
+
====Action points====
====Action points====
-
*All - Read Voygt paper  
+
*All - Read Voigt paper  
*All - Next week working off the proper wiki
*All - Next week working off the proper wiki
*Janetta contact details doc in drop box  
*Janetta contact details doc in drop box  
*Janetta and Richard – Flow chart!!!  
*Janetta and Richard – Flow chart!!!  
*Reminder to neil and jen not welcome trust funding by 2nd of April?  
*Reminder to neil and jen not welcome trust funding by 2nd of April?  
-
*Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design  
+
*Janetta - T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper, quotes dependent on design  
-
*Jannetta- send us links for graphic design software- please
+
*Jannetta - send us links for graphic design software- please
*Transfer the wiki  
*Transfer the wiki  
*Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
*Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
*Jen/Neil - Pay for team!!
*Jen/Neil - Pay for team!!
-
*Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver  
+
*Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver
 +
 
====Wiki transfer====
====Wiki transfer====
*Clarify contents of identified pages
*Clarify contents of identified pages
Line 89: Line 82:
*Identify other pages required
*Identify other pages required
*Ideas for layout/theme
*Ideas for layout/theme
-
*Zoltan Tidy it up-Urgent  
+
*Zoltan - Tidy it up - Urgent  
*Borrow last years / Look at anything thats nice  
*Borrow last years / Look at anything thats nice  
-
*It evolves!!! We can change it!!! Now!!!!!!!
+
*It evolves!!! We can change it
====Travel plans====
====Travel plans====
*Flights and Accomodation
*Flights and Accomodation
Line 99: Line 92:
*Main chemical company – free stuff?
*Main chemical company – free stuff?
====Advisors/additional team members - civil engineer/graphics designer====
====Advisors/additional team members - civil engineer/graphics designer====
-
*Still looking – Neil,Jen  
+
*Still looking – Neil, Jen
 +
 
====Tutorials====
====Tutorials====
#Sequentia and Clone manager - basics  
#Sequentia and Clone manager - basics  
Line 109: Line 103:
*Web services for Biosynthetics, Flow chart
*Web services for Biosynthetics, Flow chart
*Taverner  
*Taverner  
-
====Time line discussion====
+
====Timeline discussion====
*Need someone else on time line.  
*Need someone else on time line.  
*16th of June Lab induction  
*16th of June Lab induction  
*Deadlines soon  
*Deadlines soon  
-
*Change flight deadline – not 31st  
+
*Change flight deadline – not 31st
-
+
 
====Other business====
====Other business====
*PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up  
*PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up  

Latest revision as of 10:24, 27 October 2010

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Contents

Formal meeting - 26th March 2010

  • Room: CBCB; Start: 11 am
  • Chair: Jannetta, Minutes: Rachel, Computer: Harsh
  • Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
  • Apologies: Phil (holiday), Jem and Matt
  • Approval of agenda and minutes - Approved
  • Agenda

Research Feedback

  1. Properties of concrete and surface tension (Zoltan and Steven)
    • High compressive strength low tensile strength
    • Steal added good for temp change
    • CaCO3 – similar to concrete; expand same as concrete - might crack like concrete
    • Spider silk like steal - reduce possibility of cracking
    • Glue - no many papers - expansion may not be a problem?
    • Silk alkali resistant
    • Capillary properties of concrete change over time- in project we consider small rapid
    • Cement water ratio and surface area and environment
    • Where to look for the cracks? Crack pattern
  2. Microinjection (Zoltan and Steven)
    • Simple issue
    • Not correct term
    • Swimming Bacillus
    • Oxygen gradient big selling points
  3. Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)
    • Test in plasmids- Find good plasmid
    • Integrate into genome
    • Recombination site... Find!!!
    • amyE site amylase with insert can’t break down amylase
  4. Nutrients (Harsh and Rachel)
    • Bacteria algae growth living in concrete
    • Only activate feed bacillus
    • Two hybrid
    • Biofilms exclude other not algae
    • Antifungals, antialgaes, antibiotic
  5. Sporulation, germination and secretion control (Alan and Da)
    • SpoA – last years team sporulation control, maybe turn off completely
    • Nutrient availability
    • KinA
    • Inducible promoter
    • Glue out of bug
  6. Quorum sensing (Younus and Phil)
    • CompX and cff
    • Two-component system comP
  7. Kill switch (Richard and Jannetta)
    • Why ? Don’t want?- not die after job fill new cracks
    • Nuclease
    • Phil mazEF
    • Skf SbB
    • If linear DNA works
  8. Glue, CaCo3 and spider silk (Zoltan and Jannetta)
    • Bacteria that produce CaCo3 from urea
    • How secrete spider silk long complex proteins – how can we make it happen in bacillus
    • Secretion systems
  9. Other points
    • Linear DNA – Richard
    • Justifications of Linear
    • Build it up the use
    • Mutations
    • Not specifically a problem – keep thinking about it though

Action points

  • All - Read Voigt paper
  • All - Next week working off the proper wiki
  • Janetta contact details doc in drop box
  • Janetta and Richard – Flow chart!!!
  • Reminder to neil and jen not welcome trust funding by 2nd of April?
  • Janetta - T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper, quotes dependent on design
  • Jannetta - send us links for graphic design software- please
  • Transfer the wiki
  • Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
  • Jen/Neil - Pay for team!!
  • Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver

Wiki transfer

  • Clarify contents of identified pages
  • Ethics page
  • Research page
  • Safety page
  • Identify other pages required
  • Ideas for layout/theme
  • Zoltan - Tidy it up - Urgent
  • Borrow last years / Look at anything thats nice
  • It evolves!!! We can change it

Travel plans

  • Flights and Accomodation
    • Need to sort out who is going

Funding

  • Harsh has made draft letter
  • Main chemical company – free stuff?

Advisors/additional team members - civil engineer/graphics designer

  • Still looking – Neil, Jen

Tutorials

  1. Sequentia and Clone manager - basics
  2. Modelling part two

What next?

  • Flow chart of what we’re going to do Big picture!!!
  • If its coloured life is easier
  • Cambridge brick - Colour
  • Web services for Biosynthetics, Flow chart
  • Taverner

Timeline discussion

  • Need someone else on time line.
  • 16th of June Lab induction
  • Deadlines soon
  • Change flight deadline – not 31st

Other business

  • PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up
  • Making dropbox use better

Items for next agenda

  • Need wiki transferred by next meeting
  • Review of flow chart if done

Next meeting

  • Thursday early as possible – email to organise
  • Chair: Da, Minutes: Alan, Computer: Younus