Team:Newcastle/Meetings/26 March 2010

From 2010.igem.org

(Difference between revisions)
(4. Research Feedback)
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====4. Research Feedback====
====4. Research Feedback====
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'''Properties of concrete and surface tension''' (Zoltan and Steven)
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#'''Properties of concrete and surface tension''' (Zoltan and Steven)
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*High compressive strength low tensile strength
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#*High compressive strength low tensile strength
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*Steal added good for temp change  
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#*Steal added good for temp change  
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*CaCO<sub>3</sub> – similar to concrete; expand same as concrete... might crack like concrete  
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#*CaCO<sub>3</sub> – similar to concrete; expand same as concrete... might crack like concrete  
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*Spider silk like steal- reduce possibility of cracking   
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#*Spider silk like steal- reduce possibility of cracking   
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*Glue- no many papers- expansion may not be a problem?   
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#*Glue- no many papers- expansion may not be a problem?   
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*Silk alkali resistant  
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#*Silk alkali resistant  
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*Capillary properties of concrete change over time- in project we consider small rapid  
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#*Capillary properties of concrete change over time- in project we consider small rapid  
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*Cement water ratio and surface area and environment  
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#*Cement water ratio and surface area and environment  
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*Where to look for the cracks? Crack pattern  
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#*Where to look for the cracks? Crack pattern  
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'''Microinjection''' (Zoltan and Steven)
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#'''Microinjection''' (Zoltan and Steven)
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*Simple issue  
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#*Simple issue  
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*Not correct term  
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#*Not correct term  
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*Swimming Bacillus  
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#*Swimming Bacillus  
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*Oxygen gradient big selling points  
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#*Oxygen gradient big selling points  
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'''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel)
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#'''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel)
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*Test in plasmids- Find good plasmid  
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#*Test in plasmids- Find good plasmid  
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*Integrate into genome   
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#*Integrate into genome   
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*Recombination site... Find!!!  
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#*Recombination site... Find!!!  
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*amyE site amylase with insert can’t break down amylase  
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#*amyE site amylase with insert can’t break down amylase  
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'''Nutrients''' (Harsh and Rachel)
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#'''Nutrients''' (Harsh and Rachel)
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*Bacteria algae growth living in concrete  
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#*Bacteria algae growth living in concrete  
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*Only activate feed bacillus  
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#*Only activate feed bacillus  
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*Two hybrid
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#*Two hybrid
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*Biofilms exclude other not algae  
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#*Biofilms exclude other not algae  
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*Antifungals, antialgaes, antibiotic  
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#*Antifungals, antialgaes, antibiotic  
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'''Sporulation, germination and secretion control''' (Alan and Da)
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*SpoA – last years team sporulation control, maybe turn off completely  
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#'''Sporulation, germination and secretion control''' (Alan and Da)
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*Nutrient availability  
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#*SpoA – last years team sporulation control, maybe turn off completely  
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*KinA  
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#*Nutrient availability  
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*Inducible promoter  
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#*KinA  
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*Glue out of bug
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#*Inducible promoter  
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#*Glue out of bug
'''Quorum sensing''' (Younus and Phil)
'''Quorum sensing''' (Younus and Phil)
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Build it up the use.  
Build it up the use.  
Mutations- How stop general questions  
Mutations- How stop general questions  
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Not specifically a problem – keep thinking about it though  
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Not specifically a problem – keep thinking about it though
====Action points====
====Action points====

Revision as of 00:43, 26 October 2010

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Contents

Formal meeting - 26th March 2010

  • Room: CBCB; Start: 11 am
  • Chair: Jannetta, Minutes: Rachel, Computer: Harsh
  • Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
  • Apologies: Phil (Holiday), Jem and Matt
  • Approval of agenda and minutes - Approved

4. Research Feedback

  1. Properties of concrete and surface tension (Zoltan and Steven)
    • High compressive strength low tensile strength
    • Steal added good for temp change
    • CaCO3 – similar to concrete; expand same as concrete... might crack like concrete
    • Spider silk like steal- reduce possibility of cracking
    • Glue- no many papers- expansion may not be a problem?
    • Silk alkali resistant
    • Capillary properties of concrete change over time- in project we consider small rapid
    • Cement water ratio and surface area and environment
    • Where to look for the cracks? Crack pattern
  1. Microinjection (Zoltan and Steven)
    • Simple issue
    • Not correct term
    • Swimming Bacillus
    • Oxygen gradient big selling points
  1. Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)
    • Test in plasmids- Find good plasmid
    • Integrate into genome 
    • Recombination site... Find!!!
    • amyE site amylase with insert can’t break down amylase
  1. Nutrients (Harsh and Rachel)
    • Bacteria algae growth living in concrete
    • Only activate feed bacillus
    • Two hybrid
    • Biofilms exclude other not algae
    • Antifungals, antialgaes, antibiotic
  1. Sporulation, germination and secretion control (Alan and Da)
    • SpoA – last years team sporulation control, maybe turn off completely
    • Nutrient availability
    • KinA
    • Inducible promoter
    • Glue out of bug

Quorum sensing (Younus and Phil)

  • CompX and cff
  • Two-component system comP

Kill switch (Richard and Jannetta)

  • Why ? Don’t want?- not die after job fill new cracks
  • Nuclease
  • Phil masEF
  • Skf SbB
  • If linear DNA works


Glue, CaCo3 and spider silk (Zoltan and Jannetta)

  • Bacteria that produce CaCo3 from urea
  • How secrete spider silk long complex proteins – how can we make it happen in bacillus
  • Secretion systems

Other points

  • Linear DNA – Richard

Justifications of Linear Build it up the use. Mutations- How stop general questions Not specifically a problem – keep thinking about it though

Action points

  • All - Read Voygt paper
  • All - Next week working off the proper wiki
  • Janetta contact details doc in drop box
  • Janetta and Richard – Flow chart!!!
  • Reminder to neil and jen not welcome trust funding by 2nd of April?
  • Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design
  • Jannetta- send us links for graphic design software- please
  • Transfer the wiki
  • Individual iGEM applications - Phil hasn’t applied; Richard not accepted; Janetta text Phil
  • Jen/Neil - Pay for team!!
  • Visas/Passport update - Harsh will apply May/June; Richard needs to get a visa waiver

Wiki transfer

  • Clarify contents of identified pages
  • Ethics page
  • Research page
  • Safety page
  • Identify other pages required
  • Ideas for layout/theme
  • Zoltan Tidy it up-Urgent
  • Borrow last years / Look at anything thats nice
  • It evolves!!! We can change it!!! Now!!!!!!!

Travel plans

  • Flights and Accomodation
    • Need to sort out who is going

Funding

  • Harsh has made draft letter
  • Main chemical company – free stuff?

Advisors/additional team members - civil engineer/graphics designer

  • Still looking – Neil,Jen

Tutorials

  1. Sequentia and Clone manager - basics
  2. Modelling part two

What next?

  • Flow chart of what we’re going to do Big picture!!!
  • If its coloured life is easier
  • Cambridge brick - Colour
  • Web services for Biosynthetics, Flow chart
  • Taverner

Time line discussion

  • Need someone else on time line.
  • 16th of June Lab induction
  • Deadlines soon
  • Change flight deadline – not 31st

Other business

  • PDF mark up tools – PDF exchange viewer, foxit, keeps mark-up
  • Making dropbox use better

Items for next agenda

  • Need wiki transferred by next meeting
  • Review of flow chart if done

Next meeting

  • Thursday early as possible – email to organise
  • Chair: Da, Minutes: Alan, Computer: Younus