Team:Newcastle/Meetings/26 March 2010

From 2010.igem.org

(Difference between revisions)
Line 8: Line 8:
====4. Research Feedback====
====4. Research Feedback====
 +
'''Properties of concrete and surface tension''' (Zoltan and Steven)
'''Properties of concrete and surface tension''' (Zoltan and Steven)
*High compressive strength low tensile strength
*High compressive strength low tensile strength
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*Cement water ratio and surface area and environment  
*Cement water ratio and surface area and environment  
*Where to look for the cracks? Crack pattern  
*Where to look for the cracks? Crack pattern  
 +
'''Microinjection''' (Zoltan and Steven)
'''Microinjection''' (Zoltan and Steven)
*Simple issue  
*Simple issue  
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*Swimming Bacillus  
*Swimming Bacillus  
*Oxygen gradient big selling points  
*Oxygen gradient big selling points  
 +
'''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel)
'''Genetics Homologous recombination plasmid chromosome''' (Harsh and Rachel)
*Test in plasmids- Find good plasmid  
*Test in plasmids- Find good plasmid  
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*Recombination site... Find!!!  
*Recombination site... Find!!!  
*amyE site amylase with insert can’t break down amylase  
*amyE site amylase with insert can’t break down amylase  
 +
'''Nutrients''' (Harsh and Rachel)
'''Nutrients''' (Harsh and Rachel)
*Bacteria algae growth living in concrete  
*Bacteria algae growth living in concrete  
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*Inducible promoter  
*Inducible promoter  
*Glue out of bug
*Glue out of bug
 +
'''Quorum sensing''' (Younus and Phil)
'''Quorum sensing''' (Younus and Phil)
*CompX and cff
*CompX and cff
*Two-component system comP
*Two-component system comP
 +
'''Kill switch''' (Richard and Jannetta)
'''Kill switch''' (Richard and Jannetta)
*Why ? Don’t want?- not die after job fill new cracks  
*Why ? Don’t want?- not die after job fill new cracks  
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*If linear DNA works  
*If linear DNA works  
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=====Glue, CaCo3 and spider silk===== (Zoltan and Jannetta)
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-
Bacteria that produce CaCo3 from urea   
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'''Glue, CaCo3 and spider silk''' (Zoltan and Jannetta)
-
How secrete spider silk long complex proteins – how can we make it happen in bacillus
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*Bacteria that produce CaCo3 from urea   
-
Secretion systems  
+
*How secrete spider silk long complex proteins – how can we make it happen in bacillus
-
|-
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*Secretion systems  
-
        |9. Other points
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-
|-
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'''Other points'''
-
|Linear DNA – Richard  
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*Linear DNA – Richard  
Justifications of Linear   
Justifications of Linear   
Build it up the use.  
Build it up the use.  

Revision as of 00:30, 26 October 2010

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Formal meeting - 26th March 2010

  • Room: CBCB; Start: 11 am
  • Chair: Jannetta, Minutes: Rachel, Computer: Harsh
  • Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
  • Apologies: Phil (Holiday), Jem and Matt
  • Approval of agenda and minutes - Approved

4. Research Feedback

Properties of concrete and surface tension (Zoltan and Steven)

  • High compressive strength low tensile strength
  • Steal added good for temp change
  • CaCO3 – similar to concrete; expand same as concrete... might crack like concrete
  • Spider silk like steal- reduce possibility of cracking
  • Glue- no many papers- expansion may not be a problem?
  • Silk alkali resistant
  • Capillary properties of concrete change over time- in project we consider small rapid
  • Cement water ratio and surface area and environment
  • Where to look for the cracks? Crack pattern

Microinjection (Zoltan and Steven)

  • Simple issue
  • Not correct term
  • Swimming Bacillus
  • Oxygen gradient big selling points

Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)

  • Test in plasmids- Find good plasmid
  • Integrate into genome 
  • Recombination site... Find!!!
  • amyE site amylase with insert can’t break down amylase

Nutrients (Harsh and Rachel)

  • Bacteria algae growth living in concrete
  • Only activate feed bacillus
  • Two hybrid
  • Biofilms exclude other not algae
  • Antifungals, antialgaes, antibiotic

Sporulation, germination and secretion control (Alan and Da)

  • SpoA – last years team sporulation control, maybe turn off completely
  • Nutrient availability
  • KinA
  • Inducible promoter
  • Glue out of bug

Quorum sensing (Younus and Phil)

  • CompX and cff
  • Two-component system comP

Kill switch (Richard and Jannetta)

  • Why ? Don’t want?- not die after job fill new cracks
  • Nuclease
  • Phil masEF
  • Skf SbB
  • If linear DNA works


Glue, CaCo3 and spider silk (Zoltan and Jannetta)

  • Bacteria that produce CaCo3 from urea
  • How secrete spider silk long complex proteins – how can we make it happen in bacillus
  • Secretion systems

Other points

  • Linear DNA – Richard

Justifications of Linear Build it up the use. Mutations- How stop general questions Not specifically a problem – keep thinking about it though |-

  |5. Action points

|- |All -Read Voygt paper |- |All-Next week working off the proper wiki!!! |- |Janetta contact details doc in drop box |- |Janetta and Richard – Flow chart!!! |- |Reminder to neil and jen not welcome trust funding by 2nd of April? |- |Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design |- |Janetta- send us links for graphic design software- please |- |Transfer the wiki |-

   |1. Individual iGEM applications

|- |Phil hasn’t applied |- |Richard not accepted |- |Janetta text Phil |- |Jen/Neil-Pay for team!! |-


        |2. Visas/Passport update

|- |Harsh- May June apply |- |Richard visa waiver |-

        |3. Wiki transfer

|-

              |1. Clarify contents of identified pages

|-

              |2. Id Ethics page 

|- |Research page |- |Safety page |- |entify other pages required |-

              |3. Ideas for layout / theme

|- |Zoltan Tidy it up-Urgent |- |Borrow last years / Look at anything thats nice |- |It evolves!!! We can change it!!! Now!!!!!!! |-


    |4. Travel plans

|-

              |1. Flights

|- |Need to sort out who is going |-

              |2. Accomodation

|- |Need to sort out who is going |-

        |5. Funding

|- |Harsh has made draft letter |- |Main chemical company – free stuff ?

|-

        |6. Advisors/additional team members - civil engineer/graphics designer?

|- |Still looking –Neil,Jen |-

        |7. Tutorials

|- |Sequentia and Clone manager- basics |- |Modelling part two |-

        |8. What next?

|- |Flow chart of what we’re going to do Big picture!!! |- |If its coloured life is easier |- |Cambridge brick-Colour |- |Web services for Biosynthetics, Flow chart |- |Deverner |-

        |9. Time line discussion 

|- |Need someone else on time line. |- |16th of June Lab induction |- |Deadlines soon |- |Change flight deadline – not 31st

|-

|6. Other business

|- |Pdf mark up tools – pdf exchange viewer, foxit, keeps mark up |- |Making dropbox use better |-

  |7. Items for next agenda

|- |Need wiki transferred by next meeting!!! |- |Review of flow chart if done |-

  |8. Next meeting- Thursday early as possible – email organise 
        1. Chair-Da 
        2. Minutes-Alan
        3. Computer-Younus
|-