Team:Newcastle/Meetings/26 July 2010

Formal Meeting - 26th July 2010

• Agenda

Roll call

• Everyone present except Da, Prof. Colin Harwood, Dr Colin Davie and Dr Wendy Smith

Research feedback

1. Levan glue - Levan requires sucrose to work
   • Test how much sucrose is needed - Phil
2. Kill switch - Use of mazE and mazF
   • How much sucrose is needed for it to work?
   • How fast does the bug use up all the sucrose?
3. Stochastic switch - Use of hinC as the invertase
   • Matt to work with Phil to work out the probability of the switching
   • Have to work out what is wrong with the iGEM 2009 stochastic switch first
4. Gibson protocol
   • rocF to require 6 pair of primers, therefore try doing up 2 fragments first
5. Protocol for yneA
   • Do up the characterization separately, one bacteria to contain yneA to show filamentous, the other to have the lacI
   • Could have nice photo with the different morphology on agar
   • Do not shine UV light on during the gel extraction step to avoid DNA mutation
   • Make up lots of DNA to allow better transformation

Lab feedback

1. Arginine test - Alan and Harsh
   • Test worked fine.
   • Could refine the test by using universal liquid pH indicator or to use the pH meter
2. DNA extraction - Harsh and Racheal
   • Could only confirm the result on 27.07.2010

Wiki

• Not able to do the drop down menu with the shadow test, therefore could try using htm5
• Horizontal strip to change to purple

Visas

• Deena to travel to London on 12.08.2010

Action point

• Talk to Louela - Phil and Harsh
• Email to iGEM HQ to get stickers and badges - Harsh
• Hyperlink the protocol page to the lab notebook
• Lab wiki checker - Alan and Harsh
• Send reminder to Jen to add Jane to the mailing list - Jannetta
• Put CBCB and Biomedical school logo onto the wiki - Janetta
• Write to Chris Voigt to get the source code - Steven
• Tutorial for Inkscape - Jannetta
• To check if the extra USA visa immigration form is valid for 2 years - Jen
• Remind Neil about the MrGene logo - Steven

Next meeting

• Chair: Phil, Computer: Alan, Minutes: Rachel
• Next Wednesday, 3pm