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Team:Newcastle/Meetings/15 September 2010
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==Modelling== | ==Modelling== | ||
Flux balance analysis on wiki by next week | Flux balance analysis on wiki by next week | ||
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| + | ==By Tomorrow!== | ||
| + | * Write an abstract assuming it all worked. Email to the list by tomorrow night. Steven and Janetta. 200 words. The problem, what's been done before, what we did, what we found. Earthquakes, cracks. We will present our results at iGEM. Only Jen can submit this. | ||
| + | * Track selection. Manufacturing as main choice and environment as backup? But check all the tracks first. Then email the mailing list. Harsh will do this. Only Jen can submit this. | ||
| + | * Team roster. Everyone who is not registered needs to be. Then people selected from the pool. | ||
==Action points== | ==Action points== | ||
Revision as of 09:15, 15 September 2010
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Contents |
Roll calls
- Apologies: Phil and Deena
- Absence: Colin Harwood, Colin Davie, Da Ye, Wendy
- Concrete has been made, set in tubes.
- We went to the EM facility, won't be a problem to take photos of our concrete.
- Wiki design should be ready soon (Harsh's graphic designer friend).
Lab feedback
- pMutin4 strain transformation is not going very well. Don't just sit and wait to see if Bacillus transformation worked, do another transformation on the assumption that it hasn't.
- Do chromosomal preps from both strains (168 and 168 with pMutin4), transform with the chromosome from the opposite strain.
- As an alternative approach, Jem's LacI plasmid is here, details will be given on a sheet.
- SR1 transformed. Check that it is coming in pSB1C3 too.
- Hyperspank
- fluoresence reader and microscope
- Fluostar (Phil Aldridge/Richard?)
- Transformations of LacI E. coli strain today
- Hyperspankoid in pSB1C3
- Arabinose repressor in pSB1AK3. Has to go into BS.
Concrete
- Try
- Need to make the levans plates with "iGEM" letters
- Try to stick our concrete sticks together
Modelling
Flux balance analysis on wiki by next week
By Tomorrow!
- Write an abstract assuming it all worked. Email to the list by tomorrow night. Steven and Janetta. 200 words. The problem, what's been done before, what we did, what we found. Earthquakes, cracks. We will present our results at iGEM. Only Jen can submit this.
- Track selection. Manufacturing as main choice and environment as backup? But check all the tracks first. Then email the mailing list. Harsh will do this. Only Jen can submit this.
- Team roster. Everyone who is not registered needs to be. Then people selected from the pool.
Action points
- Neil and Jen - To register the team.
- Alan - To email Jen about the IPTG plasmid and the letters for the sucrose plates.
- Harsh - To crack the concrete into smaller pieces.
- Wendy - To talk to the EM people.
Next meeting
- Chair: Jannetta, Minutes: Steven, Computer: Alan
- Wednesday morning, 9am, CBCB.
   
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