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Team:Newcastle/Glycerol stocks
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* 1.5ml microcentrifuge tubes | * 1.5ml microcentrifuge tubes | ||
| - | * | + | * Glycerol |
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==Protocol== | ==Protocol== | ||
# Inoculate 5ml THY Broth with a single colony and incubate, stationary at 37°C for 8 hours. | # Inoculate 5ml THY Broth with a single colony and incubate, stationary at 37°C for 8 hours. | ||
| - | # 0. | + | # 0.5ml culture mixed with 0.5ml of sterile 50% glycerol (filter sterlise). |
| + | # Store at -80°C in microcentrifuge tubes. | ||
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'''Go back to our [[Team:Newcastle/Protocol list|Protocol List]]''' | '''Go back to our [[Team:Newcastle/Protocol list|Protocol List]]''' | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Latest revision as of 10:28, 19 September 2010
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Making glycerol stocks
Materials
- 1.5ml microcentrifuge tubes
- Glycerol
Protocol
- Inoculate 5ml THY Broth with a single colony and incubate, stationary at 37°C for 8 hours.
- 0.5ml culture mixed with 0.5ml of sterile 50% glycerol (filter sterlise).
- Store at -80°C in microcentrifuge tubes.
Go back to our Protocol List
   
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