Team:Newcastle/Gel electrophoresis

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(Difference between revisions)
(New page: {{Team:Newcastle/mainbanner}} ===Gel electrophoresis=== *Transfer harden gel into the gel tank *add TAE buffer until the gel is completely submerged *Depending on the nature of the samp...)
(Gel electrophoresis)
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===Gel electrophoresis===  
===Gel electrophoresis===  
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*Transfer harden gel into the gel tank
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==Materials==
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*add TAE buffer until the gel is completely submerged
+
*
-
*Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder
+
 
-
*Loading buffer was then added together with the sample before loading onto the gel matrix
+
==Procedures==
-
*Run gel at 90V until separation is achieved and visualize using the gelDoc
+
* Make up 1% agarose gel (1 g of agarose in 100 ml of TAE buffer) and transfer 60 ml of molten agarose gel into the gel tray with 3 μl of Safeview
 +
* Wait for 30 min to allow the gel to harden
 +
* Transfer harden gel into the gel tank and add TAE buffer until the gel is completely submerge
 +
* Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder was used for analysing DNA
 +
* Loading buffer was then added together with the sample before loading onto the gel matrix
 +
* Run gel at 90V until separation is achieved and visualize using the gelDoc

Revision as of 14:36, 28 July 2010

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Gel electrophoresis

Materials

Procedures

  • Make up 1% agarose gel (1 g of agarose in 100 ml of TAE buffer) and transfer 60 ml of molten agarose gel into the gel tray with 3 μl of Safeview
  • Wait for 30 min to allow the gel to harden
  • Transfer harden gel into the gel tank and add TAE buffer until the gel is completely submerge
  • Depending on the nature of the sample, 3μl of GeneRuler™ 1kb Plus DNA Ladder was used for analysing DNA
  • Loading buffer was then added together with the sample before loading onto the gel matrix
  • Run gel at 90V until separation is achieved and visualize using the gelDoc