Team:Newcastle/DNA re-hydration

From 2010.igem.org

(Difference between revisions)
(New page: This protocol is used for the re-hydration of DNA. We mainly used this for our newly arrived primers. (Link) =Materials required= * Oligonucleotide Specification Sheet * OD 260 values * ...)
(Method)
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* Calculator (!)
* Calculator (!)
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=Method=
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=Methods=
# To begin, read the Oligonucleotide Specification Sheet to find the OD 260 values and Quantity of DNA bases for each primer/DNA
# To begin, read the Oligonucleotide Specification Sheet to find the OD 260 values and Quantity of DNA bases for each primer/DNA
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# Multiple the result by 1000
# Multiple the result by 1000
# Divide 10 by the previous result, and multiple all by 100: the end result is the volume of primer/DNA in microlitres.
# Divide 10 by the previous result, and multiple all by 100: the end result is the volume of primer/DNA in microlitres.
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# Add Pure Lab Distilled Water to make the solution 100 microlitres in total.  
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# Add Pure Lab Distilled Water to make the solution up to 100 microlitres in total.  
NOTE: Extra care must be taken when following this protocol, i.e. NO contamination.
NOTE: Extra care must be taken when following this protocol, i.e. NO contamination.

Revision as of 09:06, 11 August 2010

This protocol is used for the re-hydration of DNA. We mainly used this for our newly arrived primers. (Link)

Materials required

  • Oligonucleotide Specification Sheet
  • OD 260 values
  • Quantity of DNA bases
  • Pure Lab Distilled Water
  • Calculator (!)

Methods

  1. To begin, read the Oligonucleotide Specification Sheet to find the OD 260 values and Quantity of DNA bases for each primer/DNA
  2. Once those values are found, divide the (OD 260 value) by (Quantity of DNA bases x10).
  3. Multiple the result by 1000
  4. Divide 10 by the previous result, and multiple all by 100: the end result is the volume of primer/DNA in microlitres.
  5. Add Pure Lab Distilled Water to make the solution up to 100 microlitres in total.

NOTE: Extra care must be taken when following this protocol, i.e. NO contamination.