# Team:Newcastle/DNA re-hydration

(Difference between revisions)
 Revision as of 12:36, 26 October 2010 (view source) (→Materials required)← Older edit Revision as of 12:40, 26 October 2010 (view source) (→Methods)Newer edit → Line 15: Line 15: ==Methods== ==Methods== - # To begin, read the Oligonucleotide Specification Sheet to find the OD 260 values and Quantity of DNA bases for each primer/DNA + # To begin, read the Oligonucleotide Specification Sheet to find the OD260 values and Quantity of DNA bases for each primer/DNA. - # Once those values are found, divide the (OD 260 value) by (Quantity of DNA bases x10). + # Once those values are found, divide the (OD260 value) by (Quantity of DNA bases x10). # Multiple the result by 1000 # Multiple the result by 1000 - # Divide 10 by the previous result, and multiple all by 100: the end result is the volume of primer/DNA in microlitres. + # Divide 10 by the previous result, and multiple all by 100: the end result is the volume of primer/DNA in µl. - # Add Pure Lab Distilled Water to make the solution up to 100 microlitres in total. + # Add Pure Lab Distilled Water to make the solution up to 100 µl in total.

# DNA Re-hydration

This protocol is used for re-hydration of DNA. We used this mainly for our newly arrived primers.

## Materials required

• Oligonucleotide Specification Sheet (OSS)
• OD260 values
• Quantity of DNA in bases
• Pure Lab Distilled Water

## Methods

1. To begin, read the Oligonucleotide Specification Sheet to find the OD260 values and Quantity of DNA bases for each primer/DNA.
2. Once those values are found, divide the (OD260 value) by (Quantity of DNA bases x10).
3. Multiple the result by 1000
4. Divide 10 by the previous result, and multiple all by 100: the end result is the volume of primer/DNA in µl.
5. Add Pure Lab Distilled Water to make the solution up to 100 µl in total.