DNA Re-hydration

This protocol is used for re-hydration of DNA. We used this mainly for our newly arrived primers.

Materials required

• Oligonucleotide Specification Sheet (OSS)
• OD260 values
• Quantity of DNA bases
• Pure Lab Distilled Water
• Calculator (!)

Methods

1. To begin, read the Oligonucleotide Specification Sheet to find the OD 260 values and Quantity of DNA bases for each primer/DNA
2. Once those values are found, divide the (OD 260 value) by (Quantity of DNA bases x10).
3. Multiple the result by 1000
4. Divide 10 by the previous result, and multiple all by 100: the end result is the volume of primer/DNA in microlitres.
5. Add Pure Lab Distilled Water to make the solution up to 100 microlitres in total.

Please note:

• Extra care must be taken when following this protocol, i.e. there must be NO contamination.
• The newly arrived primer tubes have to be handled with extra caution, because they will be the main stocks which working stock solutions will be made from. Therefore, gloves have to be worn, as well as preventing any contamination. Water will be used in order to liquefy the primers and the water used will be from Pure Lab Distilled Water.