Team:Newcastle/Arginine test

From 2010.igem.org

(Difference between revisions)
(Procedure)
Line 1: Line 1:
{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
-
===Materials Required===
+
=Arginine Test=
 +
 
 +
==Materials Required==
* Plate consisting of ''Bacillus subtilis'' 168 colonies.
* Plate consisting of ''Bacillus subtilis'' 168 colonies.
* Flame (streaking) Loop
* Flame (streaking) Loop
Line 9: Line 11:
* Universal Tube
* Universal Tube
-
===Procedures===
+
==Procedures==
* Perform the experiment using aseptic technique.
* Perform the experiment using aseptic technique.
* Transfer ''B. subtilis'' 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.
* Transfer ''B. subtilis'' 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.

Revision as of 15:26, 29 July 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Arginine Test

Materials Required

  • Plate consisting of Bacillus subtilis 168 colonies.
  • Flame (streaking) Loop
  • LB media consisting arginine and ampicillin
  • Auto pipette
  • Bursen Burner
  • Universal Tube

Procedures

  • Perform the experiment using aseptic technique.
  • Transfer B. subtilis 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.
  • Transfer 1 ml of the overnight culture to another universal tube containing 4 ml of the following media:
  1. Control (1) - LB media
  2. Control (2) - LB media with 10 mM of arginine
  3. Control (3) - LB media plus B. subtilis 168
  4. Test (1) - LB media with 10 mM of arginine plus B. subtilis 168
  5. Test (2) - LB media with 10 mM of arginine plus B. subtilis 168
  • Incubate the culture at 37° C with shaking.
  • Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH measuring stick.
Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon