Team:Newcastle/Arginine test

From 2010.igem.org

(Difference between revisions)
(Procedure)
(Materials Required)
 
(7 intermediate revisions not shown)
Line 1: Line 1:
{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
-
===Materials Required===
+
=Arginine Test=
-
* Plate consisting of ''Bacillus subtilis'' 168 colonies.
+
 
-
* Flame (streaking) Loop
+
==Materials Required==
-
* LB media consisting arginine and ampicillin
+
* Plate containing ''Bacillus subtilis'' 168 colonies.
 +
* Flame (streaking) loop
 +
* LB media consisting of  arginine
* Auto pipette
* Auto pipette
* Bursen Burner
* Bursen Burner
* Universal Tube
* Universal Tube
 +
* pH indicator paper
-
===Procedures===
+
==Procedures==
* Perform the experiment using aseptic technique.
* Perform the experiment using aseptic technique.
* Transfer ''B. subtilis'' 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.
* Transfer ''B. subtilis'' 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.
Line 19: Line 22:
#Test (2) - LB media with 10 mM of arginine plus ''B. subtilis'' 168
#Test (2) - LB media with 10 mM of arginine plus ''B. subtilis'' 168
* Incubate the culture at 37° C with shaking.
* Incubate the culture at 37° C with shaking.
-
* Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH measuring stick.
+
* Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH indicator paper.
 +
 
 +
 
 +
'''Go back to our [[Team:Newcastle/Protocol list|Protocol List]]'''
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 20:58, 27 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Arginine Test

Materials Required

  • Plate containing Bacillus subtilis 168 colonies.
  • Flame (streaking) loop
  • LB media consisting of arginine
  • Auto pipette
  • Bursen Burner
  • Universal Tube
  • pH indicator paper

Procedures

  • Perform the experiment using aseptic technique.
  • Transfer B. subtilis 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.
  • Transfer 1 ml of the overnight culture to another universal tube containing 4 ml of the following media:
  1. Control (1) - LB media
  2. Control (2) - LB media with 10 mM of arginine
  3. Control (3) - LB media plus B. subtilis 168
  4. Test (1) - LB media with 10 mM of arginine plus B. subtilis 168
  5. Test (2) - LB media with 10 mM of arginine plus B. subtilis 168
  • Incubate the culture at 37° C with shaking.
  • Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH indicator paper.


Go back to our Protocol List

Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon