Team:Newcastle/7 July 2010


Revision as of 11:08, 2 August 2010 by PhilipHall (Talk | contribs)

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map


We used Gel Electrophoresis to test whether we have the DNA we wanted. As we already know, the ara gene is about 300bps. We used hundred bp DNA ladder as a guidance for our bands. The two bands produced were from two separate ara cultures and they indeed show the bands in the same region, i.e. 300bps.

Newcastle PCR gel.jpg


The aim of this whole experiment was to extract genomic DNA from B. subtilis strain ATCC 6633. In order to test whether we had extracted the DNA, we first used PCR to amplify and then used Gel Electrophoresis to compare the bands of ara genes to the hundred bps ladder.It worked!

Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon