Team:Newcastle/3 September 2010


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Transformation of Bacillius subtilis 168 with pGFP-rrnB containing filamentous cell part


The aim of the experiment is to transform Bacillus subtilis 168 with the plasmid pGFP-rrnB containing our filamentous cell part. After transformation the plasmid will integrate into the chromosome at amyE. This integration removes the ability to break down starch, allowing us to select for our desired construct using the iodine test.

Filamentous in pgfprrnb.jpg

Materials and Protocol

Please refer to: Transformation of Bacillus subtilis

Note: Overnigth culture of B. subtilis 168 in MM competence medium was done the day before and the iodine test was performed the day after transformation.

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